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1eid

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{{STRUCTURE_1eid| PDB=1eid | SCENE= }}
{{STRUCTURE_1eid| PDB=1eid | SCENE= }}
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'''CRYSTAL STRUCTURE OF F120G MUTANT OF BOVINE PANCREATIC RIBONUCLEASE A'''
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===CRYSTAL STRUCTURE OF F120G MUTANT OF BOVINE PANCREATIC RIBONUCLEASE A===
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==Overview==
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The replacement of Phe120 with other hydrophobic residues causes a decrease in the activity and thermal stability in ribonuclease A (RNase A). To explain this, the crystal structures of wild-type RNase A and three mutants--F120A, F120G, and F120W--were analyzed up to a 1.4 A resolution. Although the overall backbone structures of all mutant samples were nearly the same as that of wild-type RNase A, except for the C-terminal region of F120G with a high B-factor, two local conformational changes were observed at His119 in the mutants. First, His119 of the wild-type and F120W RNase A adopted an A position, whereas those of F120A and F120G adopted a B position, but the static crystallographic position did not reflect either the efficiency of transphosphorylation or the hydrolysis reaction. Second, His119 imidazole rings of all mutant enzymes were deviated from that of wild-type RNase A, and those of F120W and F120G appeared to be "inside out" compared with that of wild-type RNase A. Only approximately 1 A change in the distance between N(epsilon2) of His12 and N(delta1) of His119 causes a drastic decrease in k(cat), indicating that the active site requires the strict positioning of the catalytic residues. A good correlation between the change in total accessible surface area of the pockets on the surface of the mutant enzymes and enthalpy change in their thermal denaturation also indicates that the effects caused by the replacements are not localized but extend to remote regions of the protein molecule.
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{{ABSTRACT_PUBMED_11742124}}
==About this Structure==
==About this Structure==
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[[Category: Ribonuclease]]
[[Category: Ribonuclease]]
[[Category: Rnase some]]
[[Category: Rnase some]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:44:49 2008''

Revision as of 21:44, 30 June 2008

Image:1eid.png

Template:STRUCTURE 1eid

CRYSTAL STRUCTURE OF F120G MUTANT OF BOVINE PANCREATIC RIBONUCLEASE A

Template:ABSTRACT PUBMED 11742124

About this Structure

1EID is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Conformational strictness required for maximum activity and stability of bovine pancreatic ribonuclease A as revealed by crystallographic study of three Phe120 mutants at 1.4 A resolution., Chatani E, Hayashi R, Moriyama H, Ueki T, Protein Sci. 2002 Jan;11(1):72-81. PMID:11742124

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