1eoh

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[[Image:1eoh.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1eoh| PDB=1eoh | SCENE= }}
{{STRUCTURE_1eoh| PDB=1eoh | SCENE= }}
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'''GLUTATHIONE TRANSFERASE P1-1'''
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===GLUTATHIONE TRANSFERASE P1-1===
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==Overview==
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An N-capping box motif (Ser/Thr-Xaa-Xaa-Asp) is strictly conserved at the beginning of helix alpha6 in the core of virtually all glutathione transferases (GST) and GST-related proteins. It has been demonstrated that this local motif is important in determining the alpha-helical propensity of the isolated alpha6-peptide and plays a crucial role in the folding and stability of GSTs. Its removal by site-directed mutagenesis generated temperature-sensitive folding mutants unable to refold at physiological temperature (37 degrees C). In the present work, variants of human GSTP1-1 (S150A and D153A), in which the capping residues have been substituted by alanine, have been generated and purified for structural analysis. Thus, for the first time, temperature-sensitive folding mutants of an enzyme, expressed at a permissive temperature, have been crystallized and their three-dimensional structures determined by X-ray crystallography. The crystal structures of human pi class GST temperature-sensitive mutants provide a basis for understanding the structural origin of the dramatic effects observed on the overall stability of the enzyme at higher temperatures upon single substitution of a capping residue.
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The line below this paragraph, {{ABSTRACT_PUBMED_10970734}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10970734 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10970734}}
==About this Structure==
==About this Structure==
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[[Category: Stenberg, G.]]
[[Category: Stenberg, G.]]
[[Category: Glutathione transferase]]
[[Category: Glutathione transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:20:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:18:38 2008''

Revision as of 22:18, 30 June 2008

Image:1eoh.png

Template:STRUCTURE 1eoh

GLUTATHIONE TRANSFERASE P1-1

Template:ABSTRACT PUBMED 10970734

About this Structure

1EOH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structures of thermolabile mutants of human glutathione transferase P1-1., Rossjohn J, McKinstry WJ, Oakley AJ, Parker MW, Stenberg G, Mannervik B, Dragani B, Cocco R, Aceto A, J Mol Biol. 2000 Sep 15;302(2):295-302. PMID:10970734

Page seeded by OCA on Tue Jul 1 01:18:38 2008

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