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1erd

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[[Image:1erd.gif|left|200px]]
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{{STRUCTURE_1erd| PDB=1erd | SCENE= }}
{{STRUCTURE_1erd| PDB=1erd | SCENE= }}
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'''THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI'''
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===THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI===
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==Overview==
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The NMR structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi has been determined in aqueous solution. The structure of this 40-residue protein was calculated with the distance geometry program DIANA from 621 distance constraints and 89 dihedral angle constraints; the program OPAL was employed for the energy minimization. For a group of 20 conformers used to characterize the solution structure, the average pairwise RMS deviation from the mean structure calculated for the backbone heavy atoms N, C alpha, and C' of residues 3-37 was 0.31 A. The molecular architecture is dominated by an up-down-up bundle of 3 short helices of residues 5-11, 14-20, and 23-33, which is similar to the structures of the homologous pheromones Er-1 and Er-10. Novel structural features include a well-defined N-cap on the first helix, a 1-residue deletion in the second helix resulting in the formation of a 3(10)-helix rather than an alpha-helix as found in Er-1 and Er-10, and the simultaneous presence of 2 different conformations for the C-terminal tetrapeptide segment, i.e., a major conformation with the Leu 39-Pro 40 peptide bond in the trans form and a minor conformation with this peptide bond in the cis form.
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(as it appears on PubMed at http://www.pubmed.gov), where 7833811 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7833811}}
==About this Structure==
==About this Structure==
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1ERD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERD OCA].
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1ERD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERD OCA].
==Reference==
==Reference==
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[[Category: Wuthrich, K.]]
[[Category: Wuthrich, K.]]
[[Category: Pheromone]]
[[Category: Pheromone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:45:23 2008''

Revision as of 22:45, 30 June 2008

Template:STRUCTURE 1erd

THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI

Template:ABSTRACT PUBMED 7833811

About this Structure

1ERD is a Single protein structure of sequence from Euplotes raikovi. Full experimental information is available from OCA.

Reference

The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi., Ottiger M, Szyperski T, Luginbuhl P, Ortenzi C, Luporini P, Bradshaw RA, Wuthrich K, Protein Sci. 1994 Sep;3(9):1515-26. PMID:7833811

Page seeded by OCA on Tue Jul 1 01:45:23 2008

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