From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:1eyo.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:1eyo.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_1eyo| PDB=1eyo | SCENE= }} | | {{STRUCTURE_1eyo| PDB=1eyo | SCENE= }} |
| | | | |
| - | '''SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA'''
| + | ===SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The three-dimensional solution structure of conotoxin TVIIA, a 30-residue polypeptide from the venom of the piscivorous cone snail Conus tulipa, has been determined using 2D 1H NMR spectroscopy. TVIIA contains six cysteine residues which form a 'four-loop' structural framework common to many peptides from Conus venoms including the omega-, delta-, kappa-, and muO-conotoxins. However, TVIIA does not belong to these well-characterized pharmacological classes of conotoxins, but displays high sequence identity with conotoxin GS, a muscle sodium channel blocker from Conus geographus. Structure calculations were based on 562 interproton distance restraints inferred from NOE data, together with 18 backbone and nine side-chain torsion angle restraints derived from spin-spin coupling constants. The final family of 20 structures had mean pairwise rms differences over residues 2-27 of 0.18+/-0.05 A for the backbone atoms and 1.39+/-0.33 A for all heavy atoms. The structure consists of a triple-stranded, antiparallel beta sheet with +2x, -1 topology (residues 7-9, 16-20 and 23-27) and several beta turns. The core of the molecule is formed by three disulfide bonds which form a cystine knot motif common to many toxic and inhibitory polypeptides. The global fold, molecular shape and distribution of amino-acid sidechains in TVIIA is similar to that previously reported for conotoxin GS, and comparison with other four-loop conotoxin structures provides further indication that TVIIA and GS represent a new and distinct subgroup of this structural family. The structure of TVIIA determined in this study provides the basis for determining a structure-activity relationship for these molecules and their interaction with target receptors. | + | The line below this paragraph, {{ABSTRACT_PUBMED_10903497}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10903497 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_10903497}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| - | 1EYO is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYO OCA]. | + | 1EYO is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYO OCA]. |
| | | | |
| | ==Reference== | | ==Reference== |
| Line 25: |
Line 29: |
| | [[Category: Hill, J M.]] | | [[Category: Hill, J M.]] |
| | [[Category: Cystine knot motif]] | | [[Category: Cystine knot motif]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:40:43 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:13:18 2008'' |
Revision as of 23:13, 30 June 2008
Template:STRUCTURE 1eyo
SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA
Template:ABSTRACT PUBMED 10903497
About this Structure
1EYO is a Single protein structure. Full experimental information is available from OCA.
Reference
Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure., Hill JM, Alewood PF, Craik DJ, Eur J Biochem. 2000 Aug;267(15):4649-57. PMID:10903497
Page seeded by OCA on Tue Jul 1 02:13:18 2008
