1f4j

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{{STRUCTURE_1f4j| PDB=1f4j | SCENE= }}
{{STRUCTURE_1f4j| PDB=1f4j | SCENE= }}
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'''STRUCTURE OF TETRAGONAL CRYSTALS OF HUMAN ERYTHROCYTE CATALASE'''
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===STRUCTURE OF TETRAGONAL CRYSTALS OF HUMAN ERYTHROCYTE CATALASE===
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==Overview==
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The structure of catalase from human erythrocytes (HEC) was determined in tetragonal crystals of space group I4(1) by molecular-replacement methods, using the orthorhombic crystal structure as a search model. It was then refined in a unit cell of dimensions a = b = 203.6 and c = 144.6 A, yielding R and R(free) of 0.196 and 0.244, respectively, for all data at 2.4 A resolution. A major difference of the HEC structure in the tetragonal crystal compared with the orthorhombic structure was the omission of a 20-residue N-terminal segment corresponding to the first exon of the human catalase gene. The overall structures were otherwise identical in both crystal forms. The NADPH-binding sites were empty in all four subunits and bound water molecules were observed at the active sites. The structure of the C-terminal segment, which corresponds to the last exon, remained undetermined. The tetragonal crystals showed a pseudo-4(1)22 symmetry in molecular packing. Two similar types of lattice contact interfaces between the HEC tetramers were observed; they were related by the pseudo-dyad axes.
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(as it appears on PubMed at http://www.pubmed.gov), where 11134921 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11134921}}
==About this Structure==
==About this Structure==
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[[Category: Heme protein]]
[[Category: Heme protein]]
[[Category: No bound nadph]]
[[Category: No bound nadph]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:53:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:40:38 2008''

Revision as of 23:40, 30 June 2008

Template:STRUCTURE 1f4j

STRUCTURE OF TETRAGONAL CRYSTALS OF HUMAN ERYTHROCYTE CATALASE

Template:ABSTRACT PUBMED 11134921

About this Structure

1F4J is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of tetragonal crystals of human erythrocyte catalase., Safo MK, Musayev FN, Wu SH, Abraham DJ, Ko TP, Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):1-7. PMID:11134921

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