1f90

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{{STRUCTURE_1f90| PDB=1f90 | SCENE= }}
{{STRUCTURE_1f90| PDB=1f90 | SCENE= }}
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'''FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE'''
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===FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE===
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==Overview==
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The three-dimensional structure of the Fab fragment of a monoclonal antibody (LNKB-2) to human interleukin-2 (IL-2) complexed with a synthetic antigenic nonapeptide, Ac-Lys-Pro-Leu-Glu-Glu-Val-Leu-Asn-Leu-OMe, has been determined at 3.0 A resolution. In the structure, four out of the six hypervariable loops of the Fab (complementarity determining regions [CDRs] L1, H1, H2, and H3) are involved in peptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. The Tyr residues in the Fab antigen binding site play a major role in antigen-antibody recognition. The structures of the complexed and uncomplexed Fab were compared. In the antigen binding site the CDR-L1 loop of the antibody shows the largest structural changes upon peptide binding. The peptide adopts a mostly alpha-helical conformation similar to that in the epitope fragment 64-72 of the IL-2 antigen. The side chains of residues Leu 66, Val 69, and Leu 70, which are shielded internally in the IL-2 structure, are involved in interactions with the Fab in the complex studied. This indicates that antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment.
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{{ABSTRACT_PUBMED_11468348}}
==About this Structure==
==About this Structure==
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[[Category: Interleukin-2]]
[[Category: Interleukin-2]]
[[Category: Monoclonal antibody]]
[[Category: Monoclonal antibody]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:53:22 2008''

Revision as of 23:53, 30 June 2008

Image:1f90.png

Template:STRUCTURE 1f90

FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE

Template:ABSTRACT PUBMED 11468348

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of an anti-interleukin-2 monoclonal antibody Fab complexed with an antigenic nonapeptide., Afonin PV, Fokin AV, Tsygannik IN, Mikhailova IY, Onoprienko LV, Mikhaleva II, Ivanov VT, Mareeva TY, Nesmeyanov VA, Li N, Pangborn WA, Duax WL, Pletnev VZ, Protein Sci. 2001 Aug;10(8):1514-21. PMID:11468348

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