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1fyh

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[[Image:1fyh.jpg|left|200px]]
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[[Image:1fyh.png|left|200px]]
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{{STRUCTURE_1fyh| PDB=1fyh | SCENE= }}
{{STRUCTURE_1fyh| PDB=1fyh | SCENE= }}
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'''1:1 COMPLEX BETWEEN AN INTERFERON GAMMA SINGLE-CHAIN VARIANT AND ITS RECEPTOR'''
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===1:1 COMPLEX BETWEEN AN INTERFERON GAMMA SINGLE-CHAIN VARIANT AND ITS RECEPTOR===
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==Overview==
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BACKGROUND: Interferon-gamma (IFN-gamma) is a homodimeric cytokine that exerts its various activities by inducing the aggregation of two different receptors. The alpha chain receptor (IFN-gammaRalpha) is a high affinity receptor that binds to IFN-gamma in a symmetric bivalent manner to form a stable, intermediate 1:2 complex. This intermediate forms a binding template for the subsequent binding of two copies of the second receptor, beta chain receptor (IFN-gammaRbeta), producing the active 1:2:2 signaling complex. RESULTS: A single chain monovalent variant of IFN-gamma (scIFN-gamma) was constructed and complexed to one copy of the extracellular domain (ECD) of IFN-gammaRalpha. The structure of this 1:1 complex was determined and the hormone-receptor interface shown to be characterized by a number of hydrophilic interactions mediated by several highly ordered water networks. The scIFN-gamma interface consists of segments from each of the monomer chains of the homodimer. The principal hydrophobic contact of the receptor involves a tripeptide segment of the receptor having an unusual and high energy conformation. Despite containing only one binding site for IFN-gammaRalpha, the monovalent scIFN-gamma molecule has significant activity in antiviral biological assays. CONCLUSIONS: ScIFN-gamma binds the ECD of IFN-gammaRalpha through a highly hydrated interface with an important set of hormone-receptor contacts mediated through structured waters. Although the interface is highly hydrated, it supports tight binding and has a considerable degree of specificity. The biological activity of scIFN-gamma confirms that the scIFN-gamma:IFN-gammaRalpha complex represents a productive intermediate and that it can effectively recruit the other required component, IFN-gammaRbeta, to signal based on the 1:1:1 complex.
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The line below this paragraph, {{ABSTRACT_PUBMED_11250200}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11250200 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11250200}}
==About this Structure==
==About this Structure==
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[[Category: Cytokine-receptor complex]]
[[Category: Cytokine-receptor complex]]
[[Category: Fibronectin type-iii]]
[[Category: Fibronectin type-iii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:54:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:07:55 2008''

Revision as of 01:07, 1 July 2008

Image:1fyh.png

Template:STRUCTURE 1fyh

1:1 COMPLEX BETWEEN AN INTERFERON GAMMA SINGLE-CHAIN VARIANT AND ITS RECEPTOR

Template:ABSTRACT PUBMED 11250200

About this Structure

1FYH is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structure and activity of a monomeric interferon-gamma:alpha-chain receptor signaling complex., Randal M, Kossiakoff AA, Structure. 2001 Feb 7;9(2):155-63. PMID:11250200

Page seeded by OCA on Tue Jul 1 04:07:55 2008

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