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1g4d

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[[Image:1g4d.gif|left|200px]]
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{{Seed}}
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[[Image:1g4d.png|left|200px]]
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{{STRUCTURE_1g4d| PDB=1g4d | SCENE= }}
{{STRUCTURE_1g4d| PDB=1g4d | SCENE= }}
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'''NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAIN/DNA COMPLEX'''
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===NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAIN/DNA COMPLEX===
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==Overview==
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We have determined the solution structure of the complex between the 'winged-helix' enhancer binding domain of the Mu repressor protein and its cognate DNA site. The structure reveals an unusual use for the 'wing' which becomes immobilized upon DNA binding where it makes intermolecular hydrogen bond contacts deep within the minor groove. Although the wing is mobile in the absence of DNA, it partially negates the large entropic penalty associated with its burial by maintaining a small degree of structural order in the DNA-free state. Extensive contacts are also formed between the recognition helix and the DNA, which reads the major groove of a highly conserved region of the binding site through a single base-specific hydrogen bond and van der Waals contacts.
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The line below this paragraph, {{ABSTRACT_PUBMED_11135677}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11135677 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11135677}}
==About this Structure==
==About this Structure==
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1G4D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G4D OCA].
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1G4D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G4D OCA].
==Reference==
==Reference==
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[[Category: Virus/viral protein]]
[[Category: Virus/viral protein]]
[[Category: Winged-helix]]
[[Category: Winged-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:07:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:23:53 2008''

Revision as of 01:23, 1 July 2008

Image:1g4d.png

Template:STRUCTURE 1g4d

NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAIN/DNA COMPLEX

Template:ABSTRACT PUBMED 11135677

About this Structure

1G4D is a Single protein structure of sequence from Enterobacteria phage mu. Full experimental information is available from OCA.

Reference

The Mu repressor-DNA complex contains an immobilized 'wing' within the minor groove., Wojciak JM, Iwahara J, Clubb RT, Nat Struct Biol. 2001 Jan;8(1):84-90. PMID:11135677

Page seeded by OCA on Tue Jul 1 04:23:53 2008

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