This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1g5q

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1g5q.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1g5q.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1g5q| PDB=1g5q | SCENE= }}
{{STRUCTURE_1g5q| PDB=1g5q | SCENE= }}
-
'''EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC'''
+
===EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC===
-
==Overview==
+
<!--
-
Epidermin from Staphylococcus epidermidis Tu3298 is an antimicrobial peptide of the lantibiotic family that contains, amongst other unusual amino acids, S:-[(Z:)- 2-aminovinyl]-D-cysteine. This residue is introduced by post-translational modification of the ribosomally synthesized precursor EpiA. Modification starts with the oxidative decarboxylation of its C-terminal cysteine by the flavoprotein EpiD generating a reactive (Z:)-enethiol intermediate. We have determined the crystal structures of EpiD and EpiD H67N in complex with the substrate pentapeptide DSYTC at 2.5 A resolution. Rossmann-type monomers build up a dodecamer of 23 point symmetry with trimers disposed at the vertices of a tetrahedron. Oligomer formation is essential for binding of flavin mononucleotide and substrate, which is buried by an otherwise disordered substrate recognition clamp. A pocket for the tyrosine residue of the substrate peptide is formed by an induced fit mechanism. The substrate contacts flavin mononucleotide only via Cys-Sgamma, suggesting its oxidation as the initial step. A thioaldehyde intermediate could undergo spontaneous decarboxylation. The unusual substrate recognition mode and the type of chemical reaction performed provide insight into a novel family of flavoproteins.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11101502}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11101502 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11101502}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Beta protein]]
[[Category: Beta protein]]
[[Category: Rossman like fold]]
[[Category: Rossman like fold]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:10:12 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:29:08 2008''

Revision as of 01:29, 1 July 2008

Image:1g5q.png

Template:STRUCTURE 1g5q

EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC

Template:ABSTRACT PUBMED 11101502

About this Structure

1G5Q is a Protein complex structure of sequences from Staphylococcus epidermidis. Full crystallographic information is available from OCA.

Reference

Crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed with a pentapeptide substrate., Blaesse M, Kupke T, Huber R, Steinbacher S, EMBO J. 2000 Dec 1;19(23):6299-310. PMID:11101502

Page seeded by OCA on Tue Jul 1 04:29:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g5q"
Personal tools