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1g75

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{{STRUCTURE_1g75| PDB=1g75 | SCENE= }}
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'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATF5UCGCG): 5-FORMYLURIDINE/ ADENOSINE BASE-PAIRS IN B-DNA'''
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===MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATF5UCGCG): 5-FORMYLURIDINE/ ADENOSINE BASE-PAIRS IN B-DNA===
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==Overview==
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To investigate the role of divalent cations in crystal packing, four different crystals of a Dickerson-type dodecamer with the sequence d(CGCGAATXCGCG), containing 2'-deoxy-5-formyluridine at X, were obtained under several conditions with and without divalent cations. The crystal structures are all isomorphous. The octahedrally hydrated magnesium cations found in the major groove cement the two neighbouring duplexes along the b axis. In the Mg(2+)-free crystals, a five-membered ring of water molecules occupies the same position as the magnesium site and connects the two duplexes similarly to the hydrated Mg(2+) ion. It has been concluded that water molecules can take the place of the hydrated magnesium cation in crystallization, but the magnesium cation is more effective and gives X-ray diffraction at slightly higher resolution. In all four crystals, the 5-formyluracil residues form the canonical Watson-Crick pair with adenine residues.
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(as it appears on PubMed at http://www.pubmed.gov), where 11173500 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11173500}}
==About this Structure==
==About this Structure==
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[[Category: Formyluridine]]
[[Category: Formyluridine]]
[[Category: Modified nucleotide]]
[[Category: Modified nucleotide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:13:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:48:52 2008''

Revision as of 01:48, 1 July 2008

Image:1g75.png

Template:STRUCTURE 1g75

MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATF5UCGCG): 5-FORMYLURIDINE/ ADENOSINE BASE-PAIRS IN B-DNA

Template:ABSTRACT PUBMED 11173500

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray analysis of a DNA dodecamer containing 2'-deoxy-5-formyluridine; what is the role of magnesium cation in crystallization of Dickerson-type DNA dodecamers?, Tsunoda M, Karino N, Ueno Y, Matsuda A, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):345-8. PMID:11173500

Page seeded by OCA on Tue Jul 1 04:48:52 2008

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