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1gft

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{{STRUCTURE_1gft| PDB=1gft | SCENE= }}
{{STRUCTURE_1gft| PDB=1gft | SCENE= }}
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'''CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS'''
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===CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS===
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==Overview==
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Water molecules make a hydration structure with the network of hydrogen bonds, covering on the surface of proteins. To quantitatively estimate the contribution of the hydration structure to protein stability, a series of hydrophilic mutant human lysozymes (Val to Ser, Tyr, Asp, Asn, and Arg) modified at three different positions on the surface, which are located in the alpha-helix (Val-110), the beta-sheet (Val-2), and the loop (Val-74), were constructed. Their thermodynamic parameters of denaturation and crystal structures were examined by calorimetry and by x-ray crystallography at 100 K, respectively. The introduced polar residues made hydrogen bonds with protein atoms and/or water molecules, sometimes changing the hydration structure around the mutation site. Changes in the stability of the mutant proteins can be evaluated by a unique equation that considers the conformational changes resulting from the substitutions. Using this analysis, the relationship between the changes in the stabilities and the hydration structures for mutant human lysozymes substituted on the surface could be quantitatively estimated. The analysis indicated that the hydration structure on protein surface plays an important role in determining the conformational stability of the protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 11927576 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11927576}}
==About this Structure==
==About this Structure==
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[[Category: Stability]]
[[Category: Stability]]
[[Category: Surface]]
[[Category: Surface]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:30:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:13:15 2008''

Revision as of 02:13, 1 July 2008

Image:1gft.png

Template:STRUCTURE 1gft

CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME SUBSTITUTED AT THE SURFACE POSITIONS

Template:ABSTRACT PUBMED 11927576

About this Structure

1GFT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Positive contribution of hydration structure on the surface of human lysozyme to the conformational stability., Funahashi J, Takano K, Yamagata Y, Yutani K, J Biol Chem. 2002 Jun 14;277(24):21792-800. Epub 2002 Apr 1. PMID:11927576

Page seeded by OCA on Tue Jul 1 05:13:15 2008

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