1gl5

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{{STRUCTURE_1gl5| PDB=1gl5 | SCENE= }}
{{STRUCTURE_1gl5| PDB=1gl5 | SCENE= }}
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'''NMR STRUCTURE OF THE SH3 DOMAIN FROM THE TEC PROTEIN TYROSINE KINASE'''
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===NMR STRUCTURE OF THE SH3 DOMAIN FROM THE TEC PROTEIN TYROSINE KINASE===
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==Overview==
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Tec is the prototypic member of a family of intracellular tyrosine kinases that includes Txk, Bmx, Itk, and Btk. Tec family kinases share similarities in domain structure with Src family kinases, but one of the features that differentiates them is a proline-rich region (PRR) preceding their Src homology (SH) 3 domain. Evidence that the PRR of Itk can bind in an intramolecular fashion to its SH3 domain and the lack of a regulatory tyrosine in the C terminus indicates that Tec kinases must be regulated by a different set of intramolecular interactions to the Src kinases. We have determined the solution structure of the Tec SH3 domain and have investigated interactions with its PRR, which contains two SH3-binding sites. We demonstrate that in vitro, the Tec PRR can bind in an intramolecular fashion to the SH3. However, the affinity is lower than that for dimerization via reciprocal PRR-SH3 association. Using site-directed mutagenesis we show that both sites can bind the Tec SH3 domain; site 1 (155KTLPPAP161) binds intramolecularly, while site 2 (165KRRPPPPIPP174) cannot and binds in an intermolecular fashion. These distinct roles for the SH3 binding sites in Tec family kinases could be important for protein targeting and enzyme activation.
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(as it appears on PubMed at http://www.pubmed.gov), where 11684687 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11684687}}
==About this Structure==
==About this Structure==
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1GL5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GL5 OCA].
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1GL5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GL5 OCA].
==Reference==
==Reference==
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[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Tyrosine-protein kinase]]
[[Category: Tyrosine-protein kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:42:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:28:07 2008''

Revision as of 02:28, 1 July 2008

Image:1gl5.png

Template:STRUCTURE 1gl5

NMR STRUCTURE OF THE SH3 DOMAIN FROM THE TEC PROTEIN TYROSINE KINASE

Template:ABSTRACT PUBMED 11684687

About this Structure

1GL5 is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

The solution structure and intramolecular associations of the Tec kinase SRC homology 3 domain., Pursglove SE, Mulhern TD, Mackay JP, Hinds MG, Booker GW, J Biol Chem. 2002 Jan 4;277(1):755-62. Epub 2001 Oct 29. PMID:11684687

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