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1gqh

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{{STRUCTURE_1gqh| PDB=1gqh | SCENE= }}
{{STRUCTURE_1gqh| PDB=1gqh | SCENE= }}
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'''QUERCETIN 2,3-DIOXYGENASE IN COMPLEX WITH THE INHIBITOR KOJIC ACID'''
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===QUERCETIN 2,3-DIOXYGENASE IN COMPLEX WITH THE INHIBITOR KOJIC ACID===
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==Overview==
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The crystal structures of the copper-dependent Aspergillus japonicus quercetin 2,3-dioxygenase (2,3QD) complexed with the inhibitors diethyldithiocarbamate (DDC) and kojic acid (KOJ) are reported at 1.70 and 2.15 A resolution, respectively. Both inhibitors asymmetrically chelate the metal center and assume a common orientation in the active site cleft. Their molecular plane blocks access to the inner portion of the cavity which is lined by the side chains of residues Met51, Thr53, Phe75, Phe114, and Met123 and which is believed to bind the flavonol B-ring of the natural substrate. The binding of the inhibitors brings order into the mixed coordination observed in the native enzyme. DDC and KOJ induce a single conformation of the Glu73 side chain, although in different ways. In the presence of DDC, Glu73 is detached from the copper ion with its carboxylate moiety pointing away from the active site cavity. In contrast, when KOJ is bound, Glu73 ligates the Cu ion through its O(epsilon)(1) atom with a monodentate geometry. Compared to the native coordinating conformation, this conformation is approximately 90 degrees rotated about the chi(3) angle. This latter Glu73 conformation is compatible with the presence of a bound substrate.
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(as it appears on PubMed at http://www.pubmed.gov), where 12069585 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12069585}}
==About this Structure==
==About this Structure==
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[[Category: Dioxygenase]]
[[Category: Dioxygenase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:53:56 2008''

Revision as of 02:54, 1 July 2008

Image:1gqh.png

Template:STRUCTURE 1gqh

QUERCETIN 2,3-DIOXYGENASE IN COMPLEX WITH THE INHIBITOR KOJIC ACID

Template:ABSTRACT PUBMED 12069585

About this Structure

1GQH is a Single protein structure of sequence from Aspergillus japonicus. Full crystallographic information is available from OCA.

Reference

Functional analysis of the copper-dependent quercetin 2,3-dioxygenase. 1. Ligand-induced coordination changes probed by X-ray crystallography: inhibition, ordering effect, and mechanistic insights., Steiner RA, Kooter IM, Dijkstra BW, Biochemistry. 2002 Jun 25;41(25):7955-62. PMID:12069585

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