1gxo

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{{STRUCTURE_1gxo| PDB=1gxo | SCENE= }}
{{STRUCTURE_1gxo| PDB=1gxo | SCENE= }}
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'''MUTANT D189A OF FAMILY 10 POLYSACCHARIDE LYASE FROM CELLVIBRIO CELLULOSA IN COMPLEX WITH TRIGALATURONIC ACID'''
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===MUTANT D189A OF FAMILY 10 POLYSACCHARIDE LYASE FROM CELLVIBRIO CELLULOSA IN COMPLEX WITH TRIGALATURONIC ACID===
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==Overview==
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Enzyme-catalyzed beta-elimination of sugar uronic acids, exemplified by the degradation of plant cell wall pectins, plays an important role in a wide spectrum of biological processes ranging from the recycling of plant biomass through to pathogen virulence. The three-dimensional crystal structure of the catalytic module of a "family PL-10" polysaccharide lyase, Pel10Acm from Cellvibrio japonicus, solved at a resolution of 1.3 A, reveals a new polysaccharide lyase fold and is the first example of a polygalacturonic acid lyase that does not exhibit the "parallel beta-helix" topology. The "Michaelis" complex of an inactive mutant in association with the substrate trigalacturonate/Ca2+ reveals the catalytic machinery harnessed by this polygalacturonate lyase, which displays a stunning resemblance, presumably through convergent evolution, to the tetragalacturonic acid complex observed for a structurally unrelated polygalacturonate lyase from family PL-1. Common coordination of the -1 and +1 subsite saccharide carboxylate groups by a protein-liganded Ca2+ ion, the positioning of an arginine catalytic base in close proximity to the alpha-carbon hydrogen and numerous other conserved enzyme-substrate interactions, considered in light of mutagenesis data for both families, suggest a generic polysaccharide anti-beta-elimination mechanism.
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{{ABSTRACT_PUBMED_12221284}}
==About this Structure==
==About this Structure==
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[[Category: Mechanism]]
[[Category: Mechanism]]
[[Category: Pectate]]
[[Category: Pectate]]
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Revision as of 03:16, 1 July 2008

Image:1gxo.png

Template:STRUCTURE 1gxo

MUTANT D189A OF FAMILY 10 POLYSACCHARIDE LYASE FROM CELLVIBRIO CELLULOSA IN COMPLEX WITH TRIGALATURONIC ACID

Template:ABSTRACT PUBMED 12221284

About this Structure

1GXO is a Single protein structure of sequence from Cellvibrio japonicus. Full crystallographic information is available from OCA.

Reference

Convergent evolution sheds light on the anti-beta -elimination mechanism common to family 1 and 10 polysaccharide lyases., Charnock SJ, Brown IE, Turkenburg JP, Black GW, Davies GJ, Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12067-72. Epub 2002 Sep 9. PMID:12221284

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