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1h1y

From Proteopedia

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[[Image:1h1y.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1h1y| PDB=1h1y | SCENE= }}
{{STRUCTURE_1h1y| PDB=1h1y | SCENE= }}
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'''THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE'''
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===THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE===
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==Overview==
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Cytosolic D-ribulose-5-phosphate 3-epimerase from rice was crystallized after EDTA treatment and structurally elucidated by X-ray diffraction to 1.9A resolution. A prominent Zn(2+) site at the active center was established in a soaking experiment. The structure was compared with that of the EDTA-treated crystalline enzyme from the chloroplasts of potato plant leaves showing some structural differences, in particular the "closed" state of a strongly conserved mobile loop covering the substrate at its putative binding site. The previous proposal for the active center was confirmed and the most likely substrate binding position and conformation was derived from the locations of the bound zinc and sulfate ions and of three water molecules. Assuming that the bound zinc ion is an integral part of the enzyme, a reaction mechanism involving a well-stabilized cis-enediolate intermediate is suggested.
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The line below this paragraph, {{ABSTRACT_PUBMED_12547196}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12547196 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12547196}}
==About this Structure==
==About this Structure==
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[[Category: Isomerase]]
[[Category: Isomerase]]
[[Category: Oxidative pentose phosphate pathway]]
[[Category: Oxidative pentose phosphate pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:19:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:29:02 2008''

Revision as of 03:29, 1 July 2008

Image:1h1y.png

Template:STRUCTURE 1h1y

THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE

Template:ABSTRACT PUBMED 12547196

About this Structure

1H1Y is a Single protein structure of sequence from Oryza sativa. Full crystallographic information is available from OCA.

Reference

Structure and catalytic mechanism of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice., Jelakovic S, Kopriva S, Suss KH, Schulz GE, J Mol Biol. 2003 Feb 7;326(1):127-35. PMID:12547196

Page seeded by OCA on Tue Jul 1 06:29:02 2008

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