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1h6m

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[[Image:1h6m.gif|left|200px]]
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{{STRUCTURE_1h6m| PDB=1h6m | SCENE= }}
{{STRUCTURE_1h6m| PDB=1h6m | SCENE= }}
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'''COVALENT GLYCOSYL-ENZYME INTERMEDIATE OF HEN EGG WHITE LYSOZYME'''
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===COVALENT GLYCOSYL-ENZYME INTERMEDIATE OF HEN EGG WHITE LYSOZYME===
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==Overview==
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Hen egg-white lysozyme (HEWL) was the first enzyme to have its three-dimensional structure determined by X-ray diffraction techniques. A catalytic mechanism, featuring a long-lived oxocarbenium-ion intermediate, was proposed on the basis of model-building studies. The 'Phillips' mechanism is widely held as the paradigm for the catalytic mechanism of beta-glycosidases that cleave glycosidic linkages with net retention of configuration of the anomeric centre. Studies with other retaining beta-glycosidases, however, provide strong evidence pointing to a common mechanism for these enzymes that involves a covalent glycosyl-enzyme intermediate, as previously postulated. Here we show, in three different cases using electrospray ionization mass spectrometry, a catalytically competent covalent glycosyl-enzyme intermediate during the catalytic cycle of HEWL. We also show the three-dimensional structure of this intermediate as determined by X-ray diffraction. We formulate a general catalytic mechanism for all retaining beta-glycosidases that includes substrate distortion, formation of a covalent intermediate, and the electrophilic migration of C1 along the reaction coordinate.
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(as it appears on PubMed at http://www.pubmed.gov), where 11518970 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11518970}}
==About this Structure==
==About this Structure==
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[[Category: Lysozyme]]
[[Category: Lysozyme]]
[[Category: Mechanism]]
[[Category: Mechanism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:30:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:42:34 2008''

Revision as of 03:42, 1 July 2008

Image:1h6m.png

Template:STRUCTURE 1h6m

COVALENT GLYCOSYL-ENZYME INTERMEDIATE OF HEN EGG WHITE LYSOZYME

Template:ABSTRACT PUBMED 11518970

About this Structure

1H6M is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate., Vocadlo DJ, Davies GJ, Laine R, Withers SG, Nature. 2001 Aug 23;412(6849):835-8. PMID:11518970

Page seeded by OCA on Tue Jul 1 06:42:34 2008

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