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1htj

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{{STRUCTURE_1htj| PDB=1htj | SCENE= }}
{{STRUCTURE_1htj| PDB=1htj | SCENE= }}
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'''STRUCTURE OF THE RGS-LIKE DOMAIN FROM PDZ-RHOGEF'''
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===STRUCTURE OF THE RGS-LIKE DOMAIN FROM PDZ-RHOGEF===
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==Overview==
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BACKGROUND: The multidomain PDZ-RhoGEF is one of many known guanine nucleotide exchange factors that upregulate Rho GTPases. PDZ-RhoGEF and related family members play a critical role in a molecular signaling pathway from heterotrimeric G protein-coupled receptors to Rho proteins. A approximately 200 residue RGS-like (RGSL) domain in PDZ-RhoGEF and its homologs is responsible for the direct association with Galpha12/13 proteins. To better understand structure-function relationships, we initiated crystallographic studies of the RGSL domain from human PDZ-RhoGEF. RESULTS: A recombinant construct of the RGSL domain was expressed in Escherichia coli and purified, but it did not crystallize. Alternative constructs were designed based on a novel strategy of targeting lysine and glutamic acid residues for mutagenesis to alanine. A triple-point mutant functionally identical to the wild-type protein was crystallized, and its structure was determined by the MAD method using Se-methionine (Se-Met) incorporation. A molecular model of the RGSL domain was refined at 2.2 A resolution, revealing an all-helical tertiary fold with the mutations located at intermolecular lattice contacts. CONCLUSIONS: The first nine helices adopt a fold similar to that observed for RGS proteins, although the sequence identity with other such known structures is below 20%. The last three helices are an integral extension of the RGS fold, packing tightly against helices 3 and 4 with multiple hydrophobic interactions. Comparison with RGS proteins suggests features that are likely relevant for interaction with G proteins. Finally, we conclude that the strategy used to produce crystals was beneficial and might be applicable to other proteins resistant to crystallization.
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(as it appears on PubMed at http://www.pubmed.gov), where 11470431 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11470431}}
==About this Structure==
==About this Structure==
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[[Category: Regulator of g protein signaling]]
[[Category: Regulator of g protein signaling]]
[[Category: Rgs-like]]
[[Category: Rgs-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:39:33 2008''

Revision as of 05:39, 1 July 2008

Image:1htj.png

Template:STRUCTURE 1htj

STRUCTURE OF THE RGS-LIKE DOMAIN FROM PDZ-RHOGEF

Template:ABSTRACT PUBMED 11470431

About this Structure

1HTJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of the RGS-like domain from PDZ-RhoGEF: linking heterotrimeric g protein-coupled signaling to Rho GTPases., Longenecker KL, Lewis ME, Chikumi H, Gutkind JS, Derewenda ZS, Structure. 2001 Jul 3;9(7):559-69. PMID:11470431

Page seeded by OCA on Tue Jul 1 08:39:33 2008

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