This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1i3x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1i3x.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1i3x.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1i3x| PDB=1i3x | SCENE= }}
{{STRUCTURE_1i3x| PDB=1i3x | SCENE= }}
-
'''SOLUTION STRUCTURE OF THE A LOOP OF 23S RIBOSOMAL RNA'''
+
===SOLUTION STRUCTURE OF THE A LOOP OF 23S RIBOSOMAL RNA===
-
==Overview==
+
<!--
-
The A loop is an essential RNA component of the ribosome peptidyltransferase center that directly interacts with aminoacyl (A)-site tRNA. The A loop is highly conserved and contains a ubiquitous 2'-O-methyl ribose modification at position U2552. Here, we present the solution structure of a modified and unmodified A-loop RNA to define both the A-loop fold and the structural impact of the U2552 modification. Solution data reveal that the A-loop RNA has a compact structure that includes a noncanonical base pair between C2556 and U2552. NMR evidence is presented that the N3 position of C2556 has a shifted pKa and that protonation at C2556-N3 changes the C-U pair geometry. Our data indicate that U2552 methylation modifies the A-loop fold, in particular the dynamics and position of residues C2556 and U2555. We compare our structural data with the structure of the A loop observed in a recent 50S crystal structure [Ban, N., Nissen, P., Hansen, J., Moore, P. B. &amp; Steitz, T. A. (2000) Science 289, 905--920; Nissen, P., Hansen, J., Ban, N., Moore, P. B. &amp; Steitz, T. A. (2000) Science 289, 920--930]. The solution and crystal structures of the A loop are dramatically different, suggesting that a structural rearrangement of the A loop must occur on docking into the peptidyltransferase center. Possible roles of this docking event, the shifted pKa of C2556 and the U2552 2'-O-methylation in the mechanism of translation, are discussed.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11259644}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11259644 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11259644}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3X OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3X OCA].
==Reference==
==Reference==
Line 25: Line 29:
[[Category: A loop]]
[[Category: A loop]]
[[Category: A site]]
[[Category: A site]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:32:32 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:22:44 2008''

Revision as of 07:22, 1 July 2008

Image:1i3x.png

Template:STRUCTURE 1i3x

SOLUTION STRUCTURE OF THE A LOOP OF 23S RIBOSOMAL RNA

Template:ABSTRACT PUBMED 11259644

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of the A loop of 23S ribosomal RNA., Blanchard SC, Puglisi JD, Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3720-5. Epub 2001 Mar 20. PMID:11259644

Page seeded by OCA on Tue Jul 1 10:22:44 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i3x"
Personal tools