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1i89

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[[Image:1i89.jpg|left|200px]]
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{{STRUCTURE_1i89| PDB=1i89 | SCENE= }}
{{STRUCTURE_1i89| PDB=1i89 | SCENE= }}
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'''CHALCONE SYNTHASE (G256L)'''
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===CHALCONE SYNTHASE (G256L)===
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==Overview==
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Chalcone synthase (CHS) belongs to the family of type III polyketide synthases (PKS) that catalyze formation of structurally diverse polyketides. CHS synthesizes a tetraketide by sequential condensation of three acetyl anions derived from malonyl-CoA decarboxylation to a p-coumaroyl moiety attached to an active site cysteine. Gly256 resides on the surface of the CHS active site that is in direct contact with the polyketide chain derived from malonyl-CoA. Thus, position 256 serves as an ideal target to probe the link between cavity volume and polyketide chain-length determination in type III PKS. Functional examination of CHS G256A, G256V, G256L, and G256F mutants reveals altered product profiles from that of wild-type CHS. With p-coumaroyl-CoA as a starter molecule, the G256A and G256V mutants produce notably more tetraketide lactone. Further restrictions in cavity volume such as that seen in the G256L and G256F mutants yield increasing levels of the styrylpyrone bis-noryangonin from a triketide intermediate. X-ray crystallographic structures of the CHS G256A, G256V, G256L, and G256F mutants establish that these substitutions reduce the size of the active site cavity without significant alterations in the conformations of the polypeptide backbones. The side chain volume of position 256 influences both the number of condensation reactions during polyketide chain extension and the conformation of the triketide and tetraketide intermediates during the cyclization reaction. These results viewed in conjunction with the natural sequence variation of residue 256 suggest that rapid diversification of product specificity without concomitant loss of substantial catalytic activity in related CHS-like enzymes can occur by site-specific evolution of side chain volume at position 256.
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(as it appears on PubMed at http://www.pubmed.gov), where 11732902 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11732902}}
==About this Structure==
==About this Structure==
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[[Category: Chalcone synthase]]
[[Category: Chalcone synthase]]
[[Category: Polyketide synthase]]
[[Category: Polyketide synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:41:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:34:20 2008''

Revision as of 07:34, 1 July 2008

Image:1i89.png

Template:STRUCTURE 1i89

CHALCONE SYNTHASE (G256L)

Template:ABSTRACT PUBMED 11732902

About this Structure

1I89 is a Single protein structure of sequence from Medicago sativa. Full crystallographic information is available from OCA.

Reference

Structure-guided programming of polyketide chain-length determination in chalcone synthase., Jez JM, Bowman ME, Noel JP, Biochemistry. 2001 Dec 11;40(49):14829-38. PMID:11732902

Page seeded by OCA on Tue Jul 1 10:34:20 2008

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