This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1i8y

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1i8y.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1i8y.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1i8y| PDB=1i8y | SCENE= }}
{{STRUCTURE_1i8y| PDB=1i8y | SCENE= }}
-
'''SEMI-AUTOMATIC STRUCTURE DETERMINATION OF THE CG1 3-30 PEPTIDE BASED ON ARIA'''
+
===SEMI-AUTOMATIC STRUCTURE DETERMINATION OF THE CG1 3-30 PEPTIDE BASED ON ARIA===
-
==Overview==
+
<!--
-
Carp granulins are members of an emerging class of proteins with a sequence motif encoding a parallel stack of two to four beta-hairpins. The carp granulin-1 protein forms a stack of four beta-hairpins, whereas its amino-terminal fragment appears to adopt a very stable stack of two beta-hairpins in solution. Here we determined a refined three-dimensional structure of this peptide fragment to examine potential conformational changes compared with the full-length protein. The structures were calculated with both a traditional method and a fast semiautomated method using ambiguous NMR distance restraints. The resulting sets of structures are very similar and show that a well-defined stack of two beta-hairpins is retained in the peptide. Conformational rearrangements compensating the loss of the carboxy-terminal subdomain of the native protein are restricted to the carboxy-terminal end of the peptide, the turn connecting the two beta-hairpins, and the Tyr(21) and Tyr(25) aromatic side chains. Further removal of the Val(1) and Ile(2) residues, which are part of the first beta-hairpin and components of two major hydrophobic clusters in the two beta-hairpin structure, results in the loss of the first beta-hairpin. The second beta-hairpin, which is closely associated with the first, retains a similar but somewhat less stable conformation. The invariable presence of the second beta-hairpin and the dependence of its stability on the first beta-hairpin suggest that the stack of two beta-hairpins may be an evolutionary conserved and autonomous folding unit. In addition, the high conformational stability makes the stack of two beta-hairpins an attractive scaffold for the development of peptide-based drug candidates.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11870861}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11870861 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11870861}}
==About this Structure==
==About this Structure==
-
1I8Y is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8Y OCA].
+
1I8Y is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8Y OCA].
==Reference==
==Reference==
Line 26: Line 30:
[[Category: Vranken, W F.]]
[[Category: Vranken, W F.]]
[[Category: Beta-hairpin stack]]
[[Category: Beta-hairpin stack]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:43:11 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:35:51 2008''

Revision as of 07:35, 1 July 2008

Image:1i8y.png

Template:STRUCTURE 1i8y

SEMI-AUTOMATIC STRUCTURE DETERMINATION OF THE CG1 3-30 PEPTIDE BASED ON ARIA

Template:ABSTRACT PUBMED 11870861

About this Structure

1I8Y is a Single protein structure. Full experimental information is available from OCA.

Reference

Solution structures of a 30-residue amino-terminal domain of the carp granulin-1 protein and its amino-terminally truncated 3-30 subfragment: implications for the conformational stability of the stack of two beta-hairpins., Vranken WF, James S, Bennett HP, Ni F, Proteins. 2002 Apr 1;47(1):14-24. PMID:11870861

Page seeded by OCA on Tue Jul 1 10:35:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i8y"
Personal tools