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1i98

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{{STRUCTURE_1i98| PDB=1i98 | SCENE= }}
{{STRUCTURE_1i98| PDB=1i98 | SCENE= }}
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'''NMR ENSEMBLE OF ION-SELECTIVE LIGAND D18 FOR PLATELET INTEGRIN ALPHAIIB-BETA3'''
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===NMR ENSEMBLE OF ION-SELECTIVE LIGAND D18 FOR PLATELET INTEGRIN ALPHAIIB-BETA3===
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==Overview==
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Integrins contain a number of divalent cation binding sites that control ligand binding affinity. Ions such as Ca(2+) and Mg(2+) bind to distinct sites on integrin and can have opposing effects on ligand binding. These effects are presumably brought about by alterations of the shape of the ligand binding pocket. To gain insight into the nature of these structural differences, we probed the integrin ligand binding site with an RGD-based library of unparalleled complexity. A cysteine-constrained phage library containing six random amino acids and the RGD motif present in seven different registers was used to select for ligands that exhibit ion-selective binding to integrin alpha(IIb)beta(3). The library was used to select for peptides that bind to the integrin alpha(IIb)beta(3) preferentially in Ca(2+) versus Mg(2+). Peptides were identified which bound selectively in each ion. The Ca(2+)-selective peptides had a range of sequences, with the only obvious consensus involving a motif that had four cysteine residues bonded in a 1,4:2,3 arrangement. Interestingly though, the Mg(2+)-selective peptides exhibited a well defined consensus motif containing Cys-X-aromatic-L/G-R-G-D-hydrophobic-R-R/K-Cys. As a first step toward understanding the structural basis for this selectivity, solution NMR structures were obtained for representatives of both sets of peptides. All peptides formed turns, with the RGD motif at the apex. The Mg(2+)-selected peptides contained a unique basic patch that protrudes from the base of the turn.
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(as it appears on PubMed at http://www.pubmed.gov), where 11748219 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11748219}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I98 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I98 OCA].
==Reference==
==Reference==
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[[Category: Integrin]]
[[Category: Integrin]]
[[Category: Rgd]]
[[Category: Rgd]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:36:31 2008''

Revision as of 07:36, 1 July 2008

Image:1i98.png

Template:STRUCTURE 1i98

NMR ENSEMBLE OF ION-SELECTIVE LIGAND D18 FOR PLATELET INTEGRIN ALPHAIIB-BETA3

Template:ABSTRACT PUBMED 11748219

About this Structure

Full experimental information is available from OCA.

Reference

Selection and structure of ion-selective ligands for platelet integrin alpha IIb(beta) 3., Smith JW, Le Calvez H, Parra-Gessert L, Preece NE, Jia X, Assa-Munt N, J Biol Chem. 2002 Mar 22;277(12):10298-305. Epub 2001 Dec 17. PMID:11748219

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