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1in1

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{{STRUCTURE_1in1| PDB=1in1 | SCENE= }}
{{STRUCTURE_1in1| PDB=1in1 | SCENE= }}
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'''NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN'''
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===NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN===
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==Overview==
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BRCT (BRCA1 carboxyl terminus) domains are found in a number of DNA repair enzymes and cell cycle regulators and are believed to mediate important protein-protein interactions. The DNA ligase IIIalpha BRCT domain partners with the distal BRCT domain of the DNA repair protein XRCC1 (X1BRCTb) in the DNA base excision repair (BER) pathway. To elucidate the mechanisms by which these two domains can interact, we have determined the solution structure of human ligase IIIalpha BRCT (L3[86], residues 837-922). The structure of L3[86] consists of a beta2beta1beta3beta4 parallel sheet with a two-alpha-helix bundle packed against one face of the sheet. This fold is conserved in several proteins having a wide range of activities, including X1BRCTb [Zhang, X. D., et al. (1998) EMBO J. 17, 6404-6411]. L3[86] exists as a dimer in solution, but an insufficient number of NOE restraints precluded the determination of the homodimer structure. However, 13C isotope-filtered and hydrogen-deuterium exchange experiments indicate that the N-terminus, alpha1, the alpha1-beta2 loop, and the three residues following alpha2 are involved in forming the dimer interface, as similarly observed in the structure of X1BRCTb. NOE and dynamic data indicate that several residues (837-844) in the N-terminal region appear to interconvert between helix and random coil conformations. Further studies of other BRCT domains and of their complexes are needed to address how these proteins interact with one another, and to shed light on how mutations can lead to disruption of function and ultimately disease.
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(as it appears on PubMed at http://www.pubmed.gov), where 11683624 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11683624}}
==About this Structure==
==About this Structure==
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1IN1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IN1 OCA].
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1IN1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IN1 OCA].
==Reference==
==Reference==
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[[Category: Thornton, K H.]]
[[Category: Thornton, K H.]]
[[Category: Parallel beta sheet]]
[[Category: Parallel beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:10:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:34:52 2008''

Revision as of 10:34, 1 July 2008

Image:1in1.png

Template:STRUCTURE 1in1

NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN

Template:ABSTRACT PUBMED 11683624

About this Structure

1IN1 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure and backbone dynamics of the human DNA ligase IIIalpha BRCT domain., Krishnan VV, Thornton KH, Thelen MP, Cosman M, Biochemistry. 2001 Nov 6;40(44):13158-66. PMID:11683624

Page seeded by OCA on Tue Jul 1 13:34:52 2008

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