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1ivj

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[[Image:1ivj.gif|left|200px]]
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{{STRUCTURE_1ivj| PDB=1ivj | SCENE= }}
{{STRUCTURE_1ivj| PDB=1ivj | SCENE= }}
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'''Crystal Structure of Rat Hemeoxygenase-1 in Complex with Heme and Azide.'''
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===Crystal Structure of Rat Hemeoxygenase-1 in Complex with Heme and Azide.===
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==Overview==
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Heme oxygenase (HO) catalyzes physiological heme degradation consisting of three sequential oxidation steps that use dioxygen molecules and reducing equivalents. We determined the crystal structure of rat HO-1 in complex with heme and azide (HO-heme-N(3)(-)) at 1.9-A resolution. The azide, whose terminal nitrogen atom is coordinated to the ferric heme iron, is situated nearly parallel to the heme plane, and its other end is directed toward the alpha-meso position of the heme. Based on resonance Raman spectroscopic analysis of HO-heme bound to dioxygen, this parallel coordination mode suggests that the azide is an analog of dioxygen. The azide is surrounded by residues of the distal F-helix with only the direction to the alpha-meso carbon being open. This indicates that regiospecific oxygenation of the heme is primarily caused by the steric constraint between the dioxygen bound to heme and the F-helix. The azide interacts with Asp-140, Arg-136, and Thr-135 through a hydrogen bond network involving five water molecules on the distal side of the heme. This network, also present in HO-heme, may function in dioxygen activation in the first hydroxylation step. From the orientation of azide in HO-heme-N(3)(-), the dioxygen or hydroperoxide bound to HO-heme, the active oxygen species of the first reaction, is inferred to have a similar orientation suitable for a direct attack on the alpha-meso carbon.
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(as it appears on PubMed at http://www.pubmed.gov), where 12235152 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12235152}}
==About this Structure==
==About this Structure==
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[[Category: Alpha helix]]
[[Category: Alpha helix]]
[[Category: Di-oxygen analog complex]]
[[Category: Di-oxygen analog complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:28:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:04:25 2008''

Revision as of 11:04, 1 July 2008

Image:1ivj.png

Template:STRUCTURE 1ivj

Crystal Structure of Rat Hemeoxygenase-1 in Complex with Heme and Azide.

Template:ABSTRACT PUBMED 12235152

About this Structure

1IVJ is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of rat heme oxygenase-1 in complex with heme bound to azide. Implication for regiospecific hydroxylation of heme at the alpha-meso carbon., Sugishima M, Sakamoto H, Higashimoto Y, Omata Y, Hayashi S, Noguchi M, Fukuyama K, J Biol Chem. 2002 Nov 22;277(47):45086-90. Epub 2002 Sep 15. PMID:12235152

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