1j1p

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[[Image:1j1p.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1j1p| PDB=1j1p | SCENE= }}
{{STRUCTURE_1j1p| PDB=1j1p | SCENE= }}
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'''Crystal structure of HyHEL-10 Fv mutant LS91A complexed with hen egg white lysozyme'''
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===Crystal structure of HyHEL-10 Fv mutant LS91A complexed with hen egg white lysozyme===
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==Overview==
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To study the role of hydrogen bonding via interfacial water molecules in protein-protein interactions, we examined the interaction between hen egg white lysozyme (HEL) and its HyHEL-10 variable domain fragment (Fv) antibody. We constructed three antibody mutants (l-Y50F, l-S91A, and l-S93A) and investigated the interactions between the mutant Fvs and HEL. Isothermal titration calorimetry indicated that the mutations significantly decreased the negative enthalpy change (8-25 kJ mol(-1)), despite some offset by a favorable entropy change. X-ray crystallography demonstrated that the complexes had nearly identical structures, including the positions of the interfacial water molecules. Taken together, the isothermal titration calorimetric and x-ray crystallographic results indicate that hydrogen bonding via interfacial water enthalpically contributes to the Fv-HEL interaction despite the partial offset because of entropy loss, suggesting that hydrogen bonding stiffens the antigen-antibody complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 12444085 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12444085}}
==About this Structure==
==About this Structure==
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[[Category: Yokota, A.]]
[[Category: Yokota, A.]]
[[Category: Antigen-antibody complex]]
[[Category: Antigen-antibody complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:41:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:22:04 2008''

Revision as of 11:22, 1 July 2008

Image:1j1p.png

Template:STRUCTURE 1j1p

Crystal structure of HyHEL-10 Fv mutant LS91A complexed with hen egg white lysozyme

Template:ABSTRACT PUBMED 12444085

About this Structure

1J1P is a Protein complex structure of sequences from Gallus gallus and Mus musculus. Full crystallographic information is available from OCA.

Reference

The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL-10-HEL interaction., Yokota A, Tsumoto K, Shiroishi M, Kondo H, Kumagai I, J Biol Chem. 2003 Feb 14;278(7):5410-8. Epub 2002 Nov 19. PMID:12444085

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