1j2j

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{{STRUCTURE_1j2j| PDB=1j2j | SCENE= }}
{{STRUCTURE_1j2j| PDB=1j2j | SCENE= }}
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'''Crystal structure of GGA1 GAT N-terminal region in complex with ARF1 GTP form'''
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===Crystal structure of GGA1 GAT N-terminal region in complex with ARF1 GTP form===
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==Overview==
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GGAs are critical for trafficking soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes through interactions with TGN-sorting receptors, ADP-ribosylation factor (ARF) and clathrin. ARF-GTP bound to TGN membranes recruits its effector GGA by binding to the GAT domain, thus facilitating recognition of GGA for cargo-loaded receptors. Here we report the X-ray crystal structures of the human GGA1-GAT domain and the complex between ARF1-GTP and the N-terminal region of the GAT domain. When unbound, the GAT domain forms an elongated bundle of three a-helices with a hydrophobic core. Structurally, this domain, combined with the preceding VHS domain, resembles CALM, an AP180 homolog involved in endocytosis. In the complex with ARF1-GTP, a helix-loop-helix of the N-terminal part of GGA1-GAT interacts with the switches 1 and 2 of ARF1 predominantly in a hydrophobic manner. These data reveal a molecular mechanism underlying membrane recruitment of adaptor proteins by ARF-GTP.
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(as it appears on PubMed at http://www.pubmed.gov), where 12679809 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12679809}}
==About this Structure==
==About this Structure==
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[[Category: Wakatsuki, S.]]
[[Category: Wakatsuki, S.]]
[[Category: Protein transport]]
[[Category: Protein transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:43:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:24:18 2008''

Revision as of 11:24, 1 July 2008

Image:1j2j.png

Template:STRUCTURE 1j2j

Crystal structure of GGA1 GAT N-terminal region in complex with ARF1 GTP form

Template:ABSTRACT PUBMED 12679809

About this Structure

1J2J is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

Reference

Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport., Shiba T, Kawasaki M, Takatsu H, Nogi T, Matsugaki N, Igarashi N, Suzuki M, Kato R, Nakayama K, Wakatsuki S, Nat Struct Biol. 2003 May;10(5):386-93. PMID:12679809

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