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1j5d

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[[Image:1j5d.gif|left|200px]]
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{{STRUCTURE_1j5d| PDB=1j5d | SCENE= }}
{{STRUCTURE_1j5d| PDB=1j5d | SCENE= }}
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'''SOLUTION STRUCTURE OF OXIDIZED PARAMAGNETIC CU(II) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803-MINIMIZED AVERAGE STRUCTURE'''
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===SOLUTION STRUCTURE OF OXIDIZED PARAMAGNETIC CU(II) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803-MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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The NMR solution structure of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803 is here reported. The protein contains paramagnetic copper(II), whose electronic relaxation times are quite unfavorable for NMR solution studies. The structure has been solved on the basis of 1041 meaningful NOESY cross-peaks, 18 1D NOEs, 26 T(1) values, 96 dihedral angle constraints, and 18 H-bonds. The detection of broad hyperfine-shifted signals and their full assignment allowed the identification of the copper(II) ligands and the determination of the Cu-S-C-H dihedral angle for the coordinated cysteine. The global root-mean-square deviation from the mean structure for the solution structure family is 0.72 +/- 0.14 and 1.16 +/- 0.17 A for backbone and heavy atoms, respectively. The structure is overall quite satisfactory and represents a breakthrough, in that it includes paramagnetic copper proteins among the metalloproteins for which solution structures can be afforded. The comparison with the available X-ray structure of a triple mutant is also performed.
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(as it appears on PubMed at http://www.pubmed.gov), where 11456890 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11456890}}
==About this Structure==
==About this Structure==
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1J5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1i0y 1i0y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5D OCA].
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1J5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1i0y 1i0y]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5D OCA].
==Reference==
==Reference==
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[[Category: Vila, A J.]]
[[Category: Vila, A J.]]
[[Category: Copper protein beta barrel electron transfer]]
[[Category: Copper protein beta barrel electron transfer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:49:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:31:35 2008''

Revision as of 11:31, 1 July 2008

Image:1j5d.png

Template:STRUCTURE 1j5d

SOLUTION STRUCTURE OF OXIDIZED PARAMAGNETIC CU(II) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803-MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 11456890

About this Structure

1J5D is a Single protein structure of sequence from Synechocystis sp.. This structure supersedes the now removed PDB entry 1i0y. Full experimental information is available from OCA.

Reference

The first solution structure of a paramagnetic copper(II) protein: the case of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803., Bertini I, Ciurli S, Dikiy A, Fernandez CO, Luchinat C, Safarov N, Shumilin S, Vila AJ, J Am Chem Soc. 2001 Mar 14;123(10):2405-13. PMID:11456890

Page seeded by OCA on Tue Jul 1 14:31:35 2008

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