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1j7z

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[[Image:1j7z.gif|left|200px]]
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{{STRUCTURE_1j7z| PDB=1j7z | SCENE= }}
{{STRUCTURE_1j7z| PDB=1j7z | SCENE= }}
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'''Osmolyte Stabilization of Ribonuclease'''
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===Osmolyte Stabilization of Ribonuclease===
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==Overview==
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Osmolytes stabilize proteins to thermal and chemical denaturation. We have studied the effects of the osmolytes sarcosine, betaine, trimethylamine-N-oxide, and taurine on the structure and stability of the protein.peptide complex RNase S using x-ray crystallography and titration calorimetry, respectively. The largest degree of stabilization is achieved with 6 m sarcosine, which increases the denaturation temperatures of RNase S and S pro by 24.6 and 17.4 degrees C, respectively, at pH 5 and protects both proteins against tryptic cleavage. Four crystal structures of RNase S in the presence of different osmolytes do not offer any evidence for osmolyte binding to the folded state of the protein or any perturbation in the water structure surrounding the protein. The degree of stabilization in 6 m sarcosine increases with temperature, ranging from -0.52 kcal mol(-1) at 20 degrees C to -5.4 kcal mol(-1) at 60 degrees C. The data support the thesis that osmolytes that stabilize proteins, do so by perturbing unfolded states, which change conformation to a compact, folding competent state in the presence of osmolyte. The increased stabilization thus results from a decrease in conformational entropy of the unfolded state.
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The line below this paragraph, {{ABSTRACT_PUBMED_11373282}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11373282 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11373282}}
==About this Structure==
==About this Structure==
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[[Category: Taurine]]
[[Category: Taurine]]
[[Category: Trimethylamine-n-oxide]]
[[Category: Trimethylamine-n-oxide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:53:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:36:47 2008''

Revision as of 11:36, 1 July 2008

Image:1j7z.png

Template:STRUCTURE 1j7z

Osmolyte Stabilization of Ribonuclease

Template:ABSTRACT PUBMED 11373282

About this Structure

1J7Z is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Osmolytes stabilize ribonuclease S by stabilizing its fragments S protein and S peptide to compact folding-competent states., Ratnaparkhi GS, Varadarajan R, J Biol Chem. 2001 Aug 3;276(31):28789-98. Epub 2001 May 23. PMID:11373282

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