1kef

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{{STRUCTURE_1kef| PDB=1kef | SCENE= }}
{{STRUCTURE_1kef| PDB=1kef | SCENE= }}
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'''PDZ1 of SAP90'''
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===PDZ1 of SAP90===
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==Overview==
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The structural features of the PDZ1 domain of the synapse-associated protein SAP90 have been characterized by NMR. A comparison with the structures of the PDZ2 and PDZ3 domains of SAP90 illustrates significant differences, which may account for the unique binding properties of these homologous domains. Within the postsynaptic density, SAP90 functions as a molecular scaffold with a number of the protein-protein interactions mediated through the PDZ1 domain. Here, using fluorescence anisotropy and NMR chemical shift analysis, we have characterized the association of PDZ1 to the C-terminal peptides of the GluR6 subunit of the kainate receptor, voltage-gated K(+) channel Kv1.4, and microtubule-associate protein CRIPT, all of which are known to associate with SAP90. The latter two, which possess the consensus sequence for binding to PDZ domains (T/S-X-V-oh), have low micromolar binding affinities (1.5-15 microm). The C terminus of GluR6, RLPGKETMA-oh, lacking the consensus sequence, binds to PDZ1 of SAP90 with an affinity of 160 microm. The NMR data illustrate that although all three peptides occupy the binding groove capped by the GLGF loop of PDZ1, specific differences are present, consistent with the variation in binding affinities.
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(as it appears on PubMed at http://www.pubmed.gov), where 11744724 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11744724}}
==About this Structure==
==About this Structure==
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1KEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEF OCA].
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1KEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEF OCA].
==Reference==
==Reference==
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[[Category: Beta-sheet]]
[[Category: Beta-sheet]]
[[Category: Glgf loop]]
[[Category: Glgf loop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:12:47 2008''

Revision as of 07:12, 2 July 2008

Image:1kef.png

Template:STRUCTURE 1kef

PDZ1 of SAP90

Template:ABSTRACT PUBMED 11744724

About this Structure

1KEF is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

The PDZ1 domain of SAP90. Characterization of structure and binding., Piserchio A, Pellegrini M, Mehta S, Blackman SM, Garcia EP, Marshall J, Mierke DF, J Biol Chem. 2002 Mar 1;277(9):6967-73. Epub 2001 Dec 14. PMID:11744724

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