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1kji

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{{STRUCTURE_1kji| PDB=1kji | SCENE= }}
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'''Crystal structure of glycinamide ribonucleotide transformylase in complex with Mg-AMPPCP'''
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===Crystal structure of glycinamide ribonucleotide transformylase in complex with Mg-AMPPCP===
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==Overview==
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PurT-encoded glycinamide ribonucleotide transformylase, or PurT transformylase, functions in purine biosynthesis by catalyzing the formylation of glycinamide ribonucleotide through a catalytic mechanism requiring Mg(2+)ATP and formate. From previous x-ray diffraction analyses, it has been demonstrated that PurT transformylase from Escherichia coli belongs to the ATP-grasp superfamily of enzymes, which are characterized by three structural motifs referred to as the A-, B-, and C-domains. In all of the ATP-grasp enzymes studied to date, the adenosine nucleotide ligands are invariably wedged between the B- and C-domains, and in some cases, such as biotin carboxylase and carbamoyl phosphate synthetase, the B-domains move significantly upon nucleotide binding. Here we present a systematic and high-resolution structural investigation of PurT transformylase complexed with various adenosine nucleotides or nucleotide analogs including Mg(2+)ATP, Mg(2+)-5'-adenylylimidodiphosphate, Mg(2+)-beta,gamma-methyleneadenosine 5'-triphosphate, Mg(2+)ATPgammaS, or Mg(2+)ADP. Taken together, these studies indicate that the conformation of the so-called "T-loop," delineated by Lys-155 to Gln-165, is highly sensitive to the chemical identity of the nucleotide situated in the binding pocket. This sensitivity to nucleotide identity is in sharp contrast to that observed for the "P-loop"-containing enzymes, in which the conformation of the binding motif is virtually unchanged in the presence or absence of nucleotides.
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{{ABSTRACT_PUBMED_11953435}}
==About this Structure==
==About this Structure==
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[[Category: Nucleotide]]
[[Category: Nucleotide]]
[[Category: Purine biosynthesis]]
[[Category: Purine biosynthesis]]
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Revision as of 07:25, 2 July 2008

Image:1kji.png

Template:STRUCTURE 1kji

Crystal structure of glycinamide ribonucleotide transformylase in complex with Mg-AMPPCP

Template:ABSTRACT PUBMED 11953435

About this Structure

1KJI is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

PurT-encoded glycinamide ribonucleotide transformylase. Accommodation of adenosine nucleotide analogs within the active site., Thoden JB, Firestine SM, Benkovic SJ, Holden HM, J Biol Chem. 2002 Jun 28;277(26):23898-908. Epub 2002 Apr 12. PMID:11953435

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