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1kso

From Proteopedia

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[[Image:1kso.gif|left|200px]]
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{{STRUCTURE_1kso| PDB=1kso | SCENE= }}
{{STRUCTURE_1kso| PDB=1kso | SCENE= }}
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'''CRYSTAL STRUCTURE OF APO S100A3'''
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===CRYSTAL STRUCTURE OF APO S100A3===
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==Overview==
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S100 proteins are involved in metal-dependent intracellular signalling. Metal-free S100A3, a cysteine-rich Ca(2+)- and Zn(2+)-binding protein, has been crystallized by vapour diffusion under the strict exclusion of oxygen and in the absence of divalent metal ions. Metal binding induces large conformational changes, rendering the apo-S100A3 crystals very sensitive to various metal compounds. Therefore, the structure was solved by MIRAS phasing using potassium iodide and xenon derivatives. Iodide replaces a water molecule at the surface of the S100A3 protein, whereas xenon binds in a hydrophobic cavity at the dimer interface. Despite significant non-isomorphism, the combination of both derivatives was sufficient for structure determination. The overall apo-S100A3 structure resembles the structures of metal-free S100B and S100A6 solution structures. In contrast to the NMR structures, the EF-hand loops are well ordered in the apo-S100A3 crystal structure. In the N-terminal pseudo-EF-hand loop a water molecule occupies the position of the Ca(2+) ion. The C-terminal canonical EF-hand loop shows an extended conformation and a different helix arrangement to other S100/metal complex crystal structures.
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(as it appears on PubMed at http://www.pubmed.gov), where 12136135 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12136135}}
==About this Structure==
==About this Structure==
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[[Category: Sargent, D F.]]
[[Category: Sargent, D F.]]
[[Category: S100,ef-hand,ca2+ binding protein,zn2+ binding protein]]
[[Category: S100,ef-hand,ca2+ binding protein,zn2+ binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:07:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:54:54 2008''

Revision as of 07:54, 2 July 2008

Image:1kso.png

Template:STRUCTURE 1kso

CRYSTAL STRUCTURE OF APO S100A3

Template:ABSTRACT PUBMED 12136135

About this Structure

1KSO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Metal-free MIRAS phasing: structure of apo-S100A3., Mittl PR, Fritz G, Sargent DF, Richmond TJ, Heizmann CW, Grutter MG, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1255-61. Epub 2002, Jul 20. PMID:12136135

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