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1kuf

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{{STRUCTURE_1kuf| PDB=1kuf | SCENE= }}
{{STRUCTURE_1kuf| PDB=1kuf | SCENE= }}
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'''High-resolution Crystal Structure of a Snake Venom Metalloproteinase from Taiwan Habu'''
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===High-resolution Crystal Structure of a Snake Venom Metalloproteinase from Taiwan Habu===
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==Overview==
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The crystal structure of TM-3, a small snake-venom metalloproteinase (SVMP) isolated from Taiwan habu (Trimeresurus mucrosquamatus), was determined at 1.35 A resolution with resultant R and R(free) values of 0.181 and 0.204, respectively. The overall structure of TM-3 is an oblate ellipsoid that contains three disulfide crosslinks, Cys118-Cys197, Cys159-Cys181 and Cys161-Cys164. It exhibits the typical structural features of SVMPs and is closely related to the structure of the catalytic proteinase domain of TNFalpha-converting enzyme (TACE). In the present structure, the essential catalytic zinc ion was found to be replaced by a cadmium ion during crystallization, as revealed by atomic absorption analysis and X-ray data. This cadmium ion is bound to six ligands, including three conserved histidines and three water molecules, displaying the coordination geometry of a distorted octahedron. One of the water molecules is proposed to play the role of stabilizing the tetrahedral intermediate during the catalysis of SVMPs. The putative S'(1) specificity pocket of TM-3 is relatively shallow, in contrast to the deep pockets of adamalysin II, atrolysin C and H(2)-proteinase, but is similar to those in acutolysin A and TACE. The shallow pocket is a consequence of the presence of the non-conserved disulfide bond Cys159-Cys181 and the residue Gln174 at the bottom of the S'(1) pocket. The results indicate that the active-site structure of TM-3, among the know structures of SVMPs examined thus far, is most similar to that of TACE owing to their close disulfide configurations and the S'(1) specificity pocket.
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{{ABSTRACT_PUBMED_12077431}}
==About this Structure==
==About this Structure==
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[[Category: Yuann, J M.]]
[[Category: Yuann, J M.]]
[[Category: Alpha/beta protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:01:13 2008''

Revision as of 08:01, 2 July 2008

Image:1kuf.png

Template:STRUCTURE 1kuf

High-resolution Crystal Structure of a Snake Venom Metalloproteinase from Taiwan Habu

Template:ABSTRACT PUBMED 12077431

About this Structure

1KUF is a Single protein structure of sequence from Protobothrops mucrosquamatus. Full crystallographic information is available from OCA.

Reference

The 1.35 A structure of cadmium-substituted TM-3, a snake-venom metalloproteinase from Taiwan habu: elucidation of a TNFalpha-converting enzyme-like active-site structure with a distorted octahedral geometry of cadmium., Huang KF, Chiou SH, Ko TP, Yuann JM, Wang AH, Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1118-28. Epub 2002, Jun 20. PMID:12077431

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