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1kwf

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{{STRUCTURE_1kwf| PDB=1kwf | SCENE= }}
{{STRUCTURE_1kwf| PDB=1kwf | SCENE= }}
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'''Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate'''
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===Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate===
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==Overview==
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The crystal structure of Clostridium thermocellum endoglucanase CelA in complex with cellopentaose has been determined at 0.94 A resolution. The oligosaccharide occupies six D-glucosyl-binding subsites, three on either side of the scissile glycosidic linkage. The substrate and product of the reaction occupy different positions at the reducing end of the cleft, where an extended array of hydrogen-bonding interactions with water molecules fosters the departure of the leaving group. Severe torsional strain upon the bound substrate forces a distorted boat(2,5) B conformation for the glucosyl residue bound at subsite -1, which facilitates the formation of an oxocarbenium ion intermediate and might favor the breakage of the sugar ring concomitant with catalysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 11884144 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11884144}}
==About this Structure==
==About this Structure==
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[[Category: Protein-carbohydrate interaction]]
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[[Category: Reaction mechanism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:15:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:09:20 2008''

Revision as of 08:09, 2 July 2008

Image:1kwf.png

Template:STRUCTURE 1kwf

Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate

Template:ABSTRACT PUBMED 11884144

About this Structure

1KWF is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.

Reference

Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate., Guerin DM, Lascombe MB, Costabel M, Souchon H, Lamzin V, Beguin P, Alzari PM, J Mol Biol. 2002 Mar 8;316(5):1061-9. PMID:11884144

Page seeded by OCA on Wed Jul 2 11:09:20 2008

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