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1l0r

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{{STRUCTURE_1l0r| PDB=1l0r | SCENE= }}
{{STRUCTURE_1l0r| PDB=1l0r | SCENE= }}
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'''NMR Solution Structure of Nogalamycin Intercalation Between Co-Axially Stacked Hairpins'''
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===NMR Solution Structure of Nogalamycin Intercalation Between Co-Axially Stacked Hairpins===
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==Overview==
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Two DNA hairpin motifs (5'-GCGAAGC-3' and 5'-ACGA AGT-3'), both stabilized by a 5'-GAA loop, have been used to design novel intramolecular double hairpin structures (5'-GCGAAGCACGAAGT-3' and 5'-ACGAAGTGCG AAGC-3') in which coaxial stacking of the two hairpin components generates a double-stranded stem region effectively with a single-strand break in the middle of the sequence at either the TG or CA step between unconnected 3' and 5' terminal bases. We have investigated by NMR the conformation and dynamics of the DNA at the strand break site. We show that mutual stacking significantly enhances the stability of each hairpin. Further, the anthracycline antibiotic nogalamycin binds cleanly to the 5'-TG (5'-CA) site formed by the mutually stacked hairpins despite the break in the sugar-phosphate backbone on one strand. The complex resembles the structure of nogalamycin-DNA complexes with the drug bound at 5'-TG sites in intact duplex sequences, with pi-stacking interactions probably the single dominant stabilizing interaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 11895443 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11895443}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0R OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0R OCA].
==Reference==
==Reference==
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[[Category: Nogalamycin intercalation]]
[[Category: Nogalamycin intercalation]]
[[Category: Single strand break]]
[[Category: Single strand break]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:24:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:26:58 2008''

Revision as of 08:27, 2 July 2008

Image:1l0r.png

Template:STRUCTURE 1l0r

NMR Solution Structure of Nogalamycin Intercalation Between Co-Axially Stacked Hairpins

Template:ABSTRACT PUBMED 11895443

About this Structure

Full experimental information is available from OCA.

Reference

Drug recognition of a DNA single strand break: nogalamycin intercalation between coaxially stacked hairpins., Williams HE, Colgrave ML, Searle MS, Eur J Biochem. 2002 Mar;269(6):1726-33. PMID:11895443

Page seeded by OCA on Wed Jul 2 11:26:58 2008

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