1l1m

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[[Image:1l1m.gif|left|200px]]
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{{Seed}}
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[[Image:1l1m.png|left|200px]]
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{{STRUCTURE_1l1m| PDB=1l1m | SCENE= }}
{{STRUCTURE_1l1m| PDB=1l1m | SCENE= }}
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'''SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1'''
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===SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1===
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==Overview==
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The lac repressor-operator system is a model system for understanding protein-DNA interactions and allosteric mechanisms in gene regulation. Despite the wealth of biochemical data provided by extensive mutations of both repressor and operator, the specific recognition mechanism of the natural lac operators by lac repressor has remained elusive. Here we present the first high-resolution structure of a dimer of the DNA-binding domain of lac repressor bound to its natural operator 01. The global positioning of the dimer on the operator is dramatically asymmetric, which results in a different pattern of specific contacts between the two sites. Specific recognition is accomplished by a combination of elongation and twist by 48 degrees of the right lac subunit relative to the left one, significant rearrangement of many side chains as well as sequence-dependent deformability of the DNA. The set of recognition mechanisms involved in the lac repressor-operator system is unique among other protein-DNA complexes and presents a nice example of the adaptability that both proteins and DNA exhibit in the context of their mutual interaction.
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The line below this paragraph, {{ABSTRACT_PUBMED_12065400}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12065400 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12065400}}
==About this Structure==
==About this Structure==
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1L1M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1M OCA].
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1L1M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1M OCA].
==Reference==
==Reference==
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[[Category: Natural lac operator]]
[[Category: Natural lac operator]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:26:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:29:54 2008''

Revision as of 08:29, 2 July 2008

Image:1l1m.png

Template:STRUCTURE 1l1m

SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1

Template:ABSTRACT PUBMED 12065400

About this Structure

1L1M is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain., Kalodimos CG, Bonvin AM, Salinas RK, Wechselberger R, Boelens R, Kaptein R, EMBO J. 2002 Jun 17;21(12):2866-76. PMID:12065400

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