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1l3z

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{{STRUCTURE_1l3z| PDB=1l3z | SCENE= }}
{{STRUCTURE_1l3z| PDB=1l3z | SCENE= }}
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'''Crystal Structure Analysis of an RNA Heptamer'''
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===Crystal Structure Analysis of an RNA Heptamer===
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==Overview==
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The crystal structure of an alternating RNA heptamer r(GUAUACA) has been determined to 2.0 A resolution and refined to an R(work) of 17.1% and R(free) of 18.5% using 2797 reflections. The heptamer crystallized in the space group C222 with a unit cell of a = 25.74, b = 106.58, c = 30.26 A and two independent strands in the asymmetric unit. Each heptamer forms a duplex with its symmetry-related strand and each duplex contains six Watson-Crick base pairs and 3'-end adenosine overhangs. Therefore, two kinds of duplex (duplex 1 and duplex 2) are formed. Duplexes 1 stack on each other forming a pseudo-continuous column, which is typical of the RNA packing mode, while duplex 2 is typical of A-DNA packing with its termini in abutting interactions. Overhang adenine residues stack within the duplexes with C3'-endo sugar pucker and C2'-endo sugar pucker in duplexes 1 and 2, respectively. A Na+ ion in the crystal lattice is water bridged to two N1 atoms of symmetry-related A7 bases.
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The line below this paragraph, {{ABSTRACT_PUBMED_12595546}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12595546 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12595546}}
==About this Structure==
==About this Structure==
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[[Category: Sundaralingam, M.]]
[[Category: Sundaralingam, M.]]
[[Category: Rna]]
[[Category: Rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:31:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:39:41 2008''

Revision as of 08:39, 2 July 2008

Image:1l3z.png

Template:STRUCTURE 1l3z

Crystal Structure Analysis of an RNA Heptamer

Template:ABSTRACT PUBMED 12595546

About this Structure

Full crystallographic information is available from OCA.

Reference

The crystal structure of an alternating RNA heptamer r(GUAUACA) forming a six base-paired duplex with 3'-end adenine overhangs., Shi K, Pan B, Sundaralingam M, Nucleic Acids Res. 2003 Mar 1;31(5):1392-7. PMID:12595546

Page seeded by OCA on Wed Jul 2 11:39:41 2008

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