1mfk

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[[Image:1mfk.gif|left|200px]]
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{{Seed}}
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[[Image:1mfk.png|left|200px]]
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{{STRUCTURE_1mfk| PDB=1mfk | SCENE= }}
{{STRUCTURE_1mfk| PDB=1mfk | SCENE= }}
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'''Structure of Prokaryotic SECIS mRNA Hairpin'''
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===Structure of Prokaryotic SECIS mRNA Hairpin===
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==Overview==
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In prokaryotes, the recoding of a UGA stop codon as a selenocysteine codon requires a special elongation factor (EF) SelB and a stem-loop structure within the mRNA called a selenocysteine insertion sequence (SECIS). Here, we used NMR spectroscopy to determine the solution structure of the SECIS mRNA hairpin and characterized its interaction with the mRNA-binding domain of SelB. Our structural and biochemical data identified the conserved structural features important for binding to EF SelB within different SECIS RNA sequences. In the free SECIS mRNA structure, conserved nucleotides are strongly exposed for recognition by SelB. Binding of the C-terminal domain of SelB stabilizes the RNA secondary structure. In the protein-RNA complex, a Watson-Crick loop base-pair leaves a GpU sequence accessible for SelB recognition. This GpU sequence at the tip of the capping tetraloop and a bulge uracil five Watson-Crick base-pairs apart from the GpU are essential for interaction with SelB.
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The line below this paragraph, {{ABSTRACT_PUBMED_12421564}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12421564 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12421564}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFK OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFK OCA].
==Reference==
==Reference==
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[[Category: Rna tetraloop]]
[[Category: Rna tetraloop]]
[[Category: Selb]]
[[Category: Selb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:58:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:51:37 2008''

Revision as of 20:51, 2 July 2008

Image:1mfk.png

Template:STRUCTURE 1mfk

Structure of Prokaryotic SECIS mRNA Hairpin

Template:ABSTRACT PUBMED 12421564

About this Structure

Full experimental information is available from OCA.

Reference

Structure of prokaryotic SECIS mRNA hairpin and its interaction with elongation factor SelB., Fourmy D, Guittet E, Yoshizawa S, J Mol Biol. 2002 Nov 15;324(1):137-50. PMID:12421564

Page seeded by OCA on Wed Jul 2 23:51:37 2008

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