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1mhx

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{{STRUCTURE_1mhx| PDB=1mhx | SCENE= }}
{{STRUCTURE_1mhx| PDB=1mhx | SCENE= }}
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'''Crystal Structures of the redesigned protein G variant NuG1'''
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===Crystal Structures of the redesigned protein G variant NuG1===
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==Overview==
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We recently described two protein G variants (NuG1 and NuG2) with redesigned first hairpins that were almost twice as stable, folded 100-fold faster, and had a switched folding mechanism relative to the wild-type protein. To test the structural accuracy of our design algorithm and to provide insights to the dramatic changes in the kinetics and thermodynamics of folding, we have now determined the crystal structures of NuG1 and NuG2 to 1.8 A and 1.85 A, respectively. We find that they adopt hairpin structures that are closer to the computational models than to wild-type protein G; the RMSD of the NuG1 hairpin to the design model and the wild-type structure are 1.7 A and 5.1 A, respectively. The crystallographic B factor in the redesigned first hairpin of NuG1 is systematically higher than the second hairpin, suggesting that the redesigned region is somewhat less rigid. A second round of structure-based design yielded new variants of NuG1 and NuG2, which are further stabilized by 0.5 kcal/mole and 0.9 kcal/mole.
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(as it appears on PubMed at http://www.pubmed.gov), where 12441390 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12441390}}
==About this Structure==
==About this Structure==
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[[Category: Alpha-beta protein]]
[[Category: Alpha-beta protein]]
[[Category: Redesigned first beta-hairpin]]
[[Category: Redesigned first beta-hairpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:03:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:00:02 2008''

Revision as of 21:00, 2 July 2008

Image:1mhx.png

Template:STRUCTURE 1mhx

Crystal Structures of the redesigned protein G variant NuG1

Template:ABSTRACT PUBMED 12441390

About this Structure

1MHX is a Single protein structure of sequence from Finegoldia magna. Full crystallographic information is available from OCA.

Reference

Crystal structures and increased stabilization of the protein G variants with switched folding pathways NuG1 and NuG2., Nauli S, Kuhlman B, Le Trong I, Stenkamp RE, Teller D, Baker D, Protein Sci. 2002 Dec;11(12):2924-31. PMID:12441390

Page seeded by OCA on Thu Jul 3 00:00:02 2008

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