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3rap

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{{STRUCTURE_3rap| PDB=3rap | SCENE= }}
{{STRUCTURE_3rap| PDB=3rap | SCENE= }}
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'''The small G protein Rap2 in a non catalytic complex with GTP'''
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===The small G protein Rap2 in a non catalytic complex with GTP===
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==Overview==
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We report a novel crystal form of the small G protein Rap2A in complex with GTP which has no GTPase activity in the crystal. The asymmetric unit contains two complexes which show that a conserved switch I residue, Tyr 32, contributes an extra hydrogen bond to the gamma-phosphate of GTP as compared to related structures with GTP analogs. Since GTP is not hydrolyzed in the crystal, this interaction is unlikely to contribute to the intrinsic GTPase activity. The comparison of other G protein structures to the Rap2-GTP complex suggests that an equivalent interaction is likely to exist in their GTP form, whether unbound or bound to an effector. This interaction has to be released to allow the GAP-activated GTPase, and presumably the intrinsic GTPase activity as well. We also discuss the definition of the flexible regions and their hinges in the light of this structure and the expanding database of G protein structures. We propose that the switch I and switch II undergo either partial or complete disorder-to-order transitions according to their cellular status, thus defining a complex energy landscape comprising more than two conformational states. We observe in addition that the region connecting the switch I and switch II is flexible in Rap2 and other G proteins. This region may be important for protein-protein interactions and possibly behave as a conformational lever arm, as characterized for Arf. Taken together, these observations suggest that the structural mechanisms of small G proteins are significantly driven by entropy-based free energy changes.
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(as it appears on PubMed at http://www.pubmed.gov), where 10591105 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10591105}}
==About this Structure==
==About this Structure==
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[[Category: Ra]]
[[Category: Ra]]
[[Category: Signaling protein]]
[[Category: Signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:11:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:04:51 2008''

Revision as of 10:04, 3 July 2008

Image:3rap.png

Template:STRUCTURE 3rap

The small G protein Rap2 in a non catalytic complex with GTP

Template:ABSTRACT PUBMED 10591105

About this Structure

3RAP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of the small G protein Rap2 in a non-catalytic complex with GTP., Menetrey J, Cherfils J, Proteins. 1999 Nov 15;37(3):465-73. PMID:10591105

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