2bgz

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[[Image:2bgz.gif|left|200px]]
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{{Seed}}
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[[Image:2bgz.png|left|200px]]
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{{STRUCTURE_2bgz| PDB=2bgz | SCENE= }}
{{STRUCTURE_2bgz| PDB=2bgz | SCENE= }}
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'''ATOMIC MODEL OF THE BACTERIAL FLAGELLAR BASED ON DOCKING AN X-RAY DERIVED HOOK STRUCTURE INTO AN EM MAP.'''
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===ATOMIC MODEL OF THE BACTERIAL FLAGELLAR BASED ON DOCKING AN X-RAY DERIVED HOOK STRUCTURE INTO AN EM MAP.===
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==Overview==
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The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- and C-terminal sequences of the eight axial proteins were predicted to form interlocking alpha-domains generating an axial tube. We report on an approximately 1-nm resolution map of the hook from Salmonella typhimurium, which reveals such a tube made from interdigitated, 1-nm rod-like densities similar to those seen in maps of the filament. Atomic models for the two outer domains of the hook subunit were docked into the corresponding outermost features of the map. The N and C termini of the hook subunit fragment are positioned next to each other and face toward the axis of the hook. The placement of these termini would permit the residues missing in the fragment to form the rod-like features that form the core domain of the hook. We also fit the hook atomic model to an approximately 2-nm resolution map of the hook from Caulobacter crescentus. The hook protein sequence from C. crescentus is largely homologous to that of S. typhimurium except for a large insertion (20 kDa). According to difference maps and our fitting, this insertion is found on the outer surface of the hook, consistent with our modeling of the hook.
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The line below this paragraph, {{ABSTRACT_PUBMED_15657146}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15657146 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15657146}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
A partial atomic structure for the flagellar hook of Salmonella typhimurium., Shaikh TR, Thomas DR, Chen JZ, Samatey FA, Matsunami H, Imada K, Namba K, Derosier DJ, Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1023-8. Epub 2005 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15657146 15657146]
A partial atomic structure for the flagellar hook of Salmonella typhimurium., Shaikh TR, Thomas DR, Chen JZ, Samatey FA, Matsunami H, Imada K, Namba K, Derosier DJ, Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1023-8. Epub 2005 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15657146 15657146]
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Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism., Samatey FA, Matsunami H, Imada K, Nagashima S, Shaikh TR, Thomas DR, Chen JZ, Derosier DJ, Kitao A, Namba K, Nature. 2004 Oct 28;431(7012):1062-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15510139 15510139]
[[Category: Salmonella typhimurium]]
[[Category: Salmonella typhimurium]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Bacterial flagellar hook]]
[[Category: Bacterial flagellar hook]]
[[Category: Bacterial motiility]]
[[Category: Bacterial motiility]]
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[[Category: Bacterial motility]]
[[Category: Electron microscopy]]
[[Category: Electron microscopy]]
[[Category: Flagellum]]
[[Category: Flagellum]]
[[Category: Helical reconstruction]]
[[Category: Helical reconstruction]]
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[[Category: Model refinement,bacterial motility]]
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[[Category: Model refinement]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:16:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 10:12:47 2008''

Revision as of 07:12, 23 July 2008

Template:STRUCTURE 2bgz

ATOMIC MODEL OF THE BACTERIAL FLAGELLAR BASED ON DOCKING AN X-RAY DERIVED HOOK STRUCTURE INTO AN EM MAP.

Template:ABSTRACT PUBMED 15657146

About this Structure

2BGZ is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

A partial atomic structure for the flagellar hook of Salmonella typhimurium., Shaikh TR, Thomas DR, Chen JZ, Samatey FA, Matsunami H, Imada K, Namba K, Derosier DJ, Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1023-8. Epub 2005 Jan 18. PMID:15657146

Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism., Samatey FA, Matsunami H, Imada K, Nagashima S, Shaikh TR, Thomas DR, Chen JZ, Derosier DJ, Kitao A, Namba K, Nature. 2004 Oct 28;431(7012):1062-8. PMID:15510139

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