2ncd
From Proteopedia
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{{STRUCTURE_2ncd| PDB=2ncd | SCENE= }} | {{STRUCTURE_2ncd| PDB=2ncd | SCENE= }} | ||
| - | + | ===NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER=== | |
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| + | The line below this paragraph, {{ABSTRACT_PUBMED_9796817}}, adds the Publication Abstract to the page | ||
| + | (as it appears on PubMed at http://www.pubmed.gov), where 9796817 is the PubMed ID number. | ||
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| + | {{ABSTRACT_PUBMED_9796817}} | ||
==About this Structure== | ==About this Structure== | ||
| - | 2NCD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. | + | 2NCD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Additional information on 2NCD is available in a page on [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb64_1.html Kinesin] at the RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NCD OCA]. |
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| + | ==Reference== | ||
| + | Direction determination in the minus-end-directed kinesin motor ncd., Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ, Nature. 1998 Oct 22;395(6704):813-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9796817 9796817] | ||
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Kinesin]] | [[Category: Kinesin]] | ||
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[[Category: Ncd]] | [[Category: Ncd]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 11:13:10 2008'' |
Revision as of 08:13, 23 July 2008
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| 2ncd, resolution 2.50Å () | |||||||||
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| Ligands: | , | ||||||||
| Gene: | NCD (Drosophila melanogaster) | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER
Motor proteins of the kinesin superfamily transport intracellular cargo along microtubules. Although different kinesin proteins share 30-50% amino-acid identity in their motor catalytic cores, some move to the plus end of microtubules whereas others travel in the opposite direction. Crystal structures of the catalytic cores of conventional kinesin (a plus-end-directed motor involved in organelle transport) and ncd (a minus-end-directed motor involved in chromosome segregation) are nearly identical; therefore, the structural basis for their opposite directions of movement is unknown. Here we show that the ncd 'neck' made up of 13 class-specific residues next to the superfamily-conserved catalytic core, is essential for minus-end-directed motility, as mutagenesis of these neck residues reverses the direction of ncd motion. By solving the 2.5 A structure of a functional ncd dimer, we show that the ncd neck (a coiled-coil) differs from the corresponding region in the kinesin neck (an interrupted beta-strand), although both necks interact with similar elements in the catalytic cores. The distinct neck architectures also confer different symmetries to the ncd and kinesin dimers and position these motors with appropriate directional bias on the microtubule.
Direction determination in the minus-end-directed kinesin motor ncd., Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ, Nature. 1998 Oct 22;395(6704):813-6. PMID:9796817
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2NCD is a Single protein structure of sequence from Drosophila melanogaster. Additional information on 2NCD is available in a page on Kinesin at the RCSB PDB Molecule of the Month. Full crystallographic information is available from OCA.
Reference
Direction determination in the minus-end-directed kinesin motor ncd., Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ, Nature. 1998 Oct 22;395(6704):813-6. PMID:9796817
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