From Proteopedia
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| | {{STRUCTURE_1vtp| PDB=1vtp | SCENE= }} | | {{STRUCTURE_1vtp| PDB=1vtp | SCENE= }} |
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| - | '''VACUOLAR TARGETING PEPTIDE FROM NA-PROPI'''
| + | ===VACUOLAR TARGETING PEPTIDE FROM NA-PROPI=== |
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| - | ==Overview==
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| - | NA-proPI is a 40.3-kDa multidomain precursor protein found in the stigma of the ornamental tobacco Nicotiana alata. It is selectively targeted to the vacuole and, as the plant matures, is processed to produce a series of five 6-kDa proteinase inhibitors (one chymotrypsin and four trypsin reactive sites) which are thought to play a vital role in plant protection against insect pests. A putative sixth domain with a chymotrypsin reactive site is likely to be formed by three disulfide bridges linking the N- and C-terminal fragments of NA-proPI. This domain contains two distinct structural elements: a 54-residue sequence with high identity to each of the five repeated PI domains, and a nonrepeated 25-residue sequence at the C-terminus which is proposed to contain a vacuolar targeting peptide. The structure of the putative sixth domain was predicted using a combination of secondary structure prediction and homology modeling based on the known structure of one of the intact domains. A 26-residue peptide corresponding to the nonrepeated C-terminal sequence and encompassing the putative vacuolar targeting sequence was synthesized and its structure determined using 1H NMR spectroscopy and simulated annealing calculations. The peptide was found to adopt an amphipathic helical structure. The calculations based on NOE data suggested that the helix is curved, with a hydrophobic concave face and a hydrophilic convex face. This curvature is consistent with an observed periodicity in backbone NH chemical shifts. The structure of the entire sixth domain was modeled by combining the experimentally determined structure of the putative vacuolar targeting peptide with the homology model of the PI domain. In this model the alpha-helix of the putative targeting peptide protrudes from the otherwise compact PI domain. This observation is consistent with the requirement for targeting sequences to be relatively exposed for recognition by the sorting apparatus. As there is no consensus on the structure of vacuolar targeting sequences, this study provides a valuable insight into their potential mechanism of interaction with the cellular sorting apparatus.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8555206}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8555206 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8555206}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1VTP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VTP OCA]. | + | 1VTP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VTP OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Nielsen, K J.]] | | [[Category: Nielsen, K J.]] |
| | [[Category: Nicotiana alata proteinase inhibitor]] | | [[Category: Nicotiana alata proteinase inhibitor]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:52:54 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:33:53 2008'' |
Revision as of 11:33, 27 July 2008
Template:STRUCTURE 1vtp
VACUOLAR TARGETING PEPTIDE FROM NA-PROPI
Template:ABSTRACT PUBMED 8555206
About this Structure
1VTP is a Single protein structure of sequence from Nicotiana alata. Full experimental information is available from OCA.
Reference
Synthesis and structure determination by NMR of a putative vacuolar targeting peptide and model of a proteinase inhibitor from Nicotiana alata., Nielsen KJ, Hill JM, Anderson MA, Craik DJ, Biochemistry. 1996 Jan 16;35(2):369-78. PMID:8555206
Page seeded by OCA on Sun Jul 27 14:33:53 2008
