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1w4x

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[[Image:1w4x.gif|left|200px]]
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{{STRUCTURE_1w4x| PDB=1w4x | SCENE= }}
{{STRUCTURE_1w4x| PDB=1w4x | SCENE= }}
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'''PHENYLACETONE MONOOXYGENASE, A BAEYER-VILLIGER MONOOXYGENASE'''
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===PHENYLACETONE MONOOXYGENASE, A BAEYER-VILLIGER MONOOXYGENASE===
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==Overview==
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Flavin-containing Baeyer-Villiger monooxygenases employ NADPH and molecular oxygen to catalyze the insertion of an oxygen atom into a carbon-carbon bond of a carbonylic substrate. These enzymes can potentially be exploited in a variety of biocatalytic applications given the wide use of Baeyer-Villiger reactions in synthetic organic chemistry. The catalytic activity of these enzymes involves the formation of two crucial intermediates: a flavin peroxide generated by the reaction of the reduced flavin with molecular oxygen and the "Criegee" intermediate resulting from the attack of the flavin peroxide onto the substrate that is being oxygenated. The crystal structure of phenylacetone monooxygenase, a Baeyer-Villiger monooxygenase from the thermophilic bacterium Thermobifida fusca, exhibits a two-domain architecture resembling that of the disulfide oxidoreductases. The active site is located in a cleft at the domain interface. An arginine residue lays above the flavin ring in a position suited to stabilize the negatively charged flavin-peroxide and Criegee intermediates. This amino acid residue is predicted to exist in two positions; the "IN" position found in the crystal structure and an "OUT" position that allows NADPH to approach the flavin to reduce the cofactor. Domain rotations are proposed to bring about the conformational changes involved in catalysis. The structural studies highlight the functional complexity of this class of flavoenzymes, which coordinate the binding of three substrates (molecular oxygen, NADPH, and phenylacetone) in proximity of the flavin cofactor with formation of two distinct catalytic intermediates.
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(as it appears on PubMed at http://www.pubmed.gov), where 15328411 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15328411}}
==About this Structure==
==About this Structure==
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[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
[[Category: Oxygenase]]
[[Category: Oxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:09:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:19:45 2008''

Revision as of 12:19, 27 July 2008

Image:1w4x.png

Template:STRUCTURE 1w4x

PHENYLACETONE MONOOXYGENASE, A BAEYER-VILLIGER MONOOXYGENASE

Template:ABSTRACT PUBMED 15328411

About this Structure

1W4X is a Single protein structure of sequence from Thermobifida fusca. Full crystallographic information is available from OCA.

Reference

Crystal structure of a Baeyer-Villiger monooxygenase., Malito E, Alfieri A, Fraaije MW, Mattevi A, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13157-62. Epub 2004 Aug 24. PMID:15328411

Page seeded by OCA on Sun Jul 27 15:19:45 2008

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