2jnq

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{{STRUCTURE_2jnq| PDB=2jnq | SCENE= }}
{{STRUCTURE_2jnq| PDB=2jnq | SCENE= }}
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'''Solution Structure of a KlbA Intein Precursor from Methanococcus jannaschii'''
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===Solution Structure of a KlbA Intein Precursor from Methanococcus jannaschii===
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==Overview==
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Certain proteins of unicellular organisms are translated as precursor polypeptides containing inteins (intervening proteins), which are domains capable of performing protein splicing. These domains, in conjunction with a single residue following the intein, catalyze their own excision from the surrounding protein (extein) in a multistep reaction involving the cleavage of two intein-extein peptide bonds and the formation of a new peptide bond that ligates the two exteins to yield the mature protein. We report here the solution NMR structure of a 186-residue precursor of the KlbA intein from Methanococcus jannaschii, comprising the intein together with N- and C-extein segments of 7 and 11 residues, respectively. The intein is shown to adopt a single, well-defined globular domain, representing a HINT (Hedgehog/Intein)-type topology. Fourteen beta-strands are arranged in a complex fold that includes four beta-hairpins and an antiparallel beta-ribbon, and there is one alpha-helix, which is packed against the beta-ribbon, and one turn of 3(10)-helix in the loop between the beta-strands 8 and 9. The two extein segments show increased disorder, and form only minimal nonbonding contacts with the intein domain. Structure-based mutation experiments resulted in a proposal for functional roles of individual residues in the intein catalytic mechanism.
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{{ABSTRACT_PUBMED_17586768}}
==About this Structure==
==About this Structure==
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2JNQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNQ OCA].
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2JNQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNQ OCA].
==Reference==
==Reference==
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[[Category: Wuthrich, K A.]]
[[Category: Wuthrich, K A.]]
[[Category: Protein]]
[[Category: Protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:04:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:22:01 2008''

Revision as of 12:22, 27 July 2008

Image:2jnq.png

Template:STRUCTURE 2jnq

Solution Structure of a KlbA Intein Precursor from Methanococcus jannaschii

Template:ABSTRACT PUBMED 17586768

About this Structure

2JNQ is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full experimental information is available from OCA.

Reference

NMR structure of a KlbA intein precursor from Methanococcus jannaschii., Johnson MA, Southworth MW, Herrmann T, Brace L, Perler FB, Wuthrich K, Protein Sci. 2007 Jul;16(7):1316-28. PMID:17586768

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