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1s2f

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{{STRUCTURE_1s2f| PDB=1s2f | SCENE= }}
{{STRUCTURE_1s2f| PDB=1s2f | SCENE= }}
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'''Average solution structure of a pseudo-5'-splice site from the negative regulator of splicing of Rous Sarcoma virus'''
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===Average solution structure of a pseudo-5'-splice site from the negative regulator of splicing of Rous Sarcoma virus===
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==Overview==
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Control of Rous sarcoma virus RNA splicing depends in part on the interaction of U1 and U11 snRNPs with an intronic RNA element called the negative regulator of splicing (NRS). A 23mer RNA hairpin (NRS23) of the NRS directly binds U1 and U11 snRNPs. Mutations that disrupt base-pairing between the loop of NRS23 and U1 snRNA abolish its negative control of splicing. We have determined the solution structure of NRS23 using NOEs, torsion angles, and residual dipolar couplings that were extracted from multidimensional heteronuclear NMR spectra. Our structure showed that the 6-bp stem of NRS23 adopts a nearly A-form duplex conformation. The loop, which consists of 11 residues according to secondary structure probing, was in a closed conformation. U913, the first residue in the loop, was bulged out or dynamic, and loop residues G914-C923, G915-U922, and U916-A921 were base-paired. The remaining UUGU tetraloop sequence did not adopt a stable structure and appears flexible in solution. This tetraloop differs from the well-known classes of tetraloops (GNRA, CUYG, UNCG) in terms of its stability, structure, and function. Deletion of the bulged U913, which is not complementary to U1 snRNA, increased the melting temperature of the RNA hairpin. This hyperstable hairpin exhibited a significant decrease in binding to U1 snRNP. Thus, the structure of the NRS RNA, as well as its sequence, is important for interaction with U1 snRNP and for splicing suppression.
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The line below this paragraph, {{ABSTRACT_PUBMED_15317975}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15317975 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15317975}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2F OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2F OCA].
==Reference==
==Reference==
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[[Category: Nr]]
[[Category: Nr]]
[[Category: U1 snrnp binding site]]
[[Category: U1 snrnp binding site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:12:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:30:52 2008''

Revision as of 12:31, 27 July 2008

Image:1s2f.png

Template:STRUCTURE 1s2f

Average solution structure of a pseudo-5'-splice site from the negative regulator of splicing of Rous Sarcoma virus

Template:ABSTRACT PUBMED 15317975

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of the pseudo-5' splice site of a retroviral splicing suppressor., Cabello-Villegas J, Giles KE, Soto AM, Yu P, Mougin A, Beemon KL, Wang YX, RNA. 2004 Sep;10(9):1388-98. PMID:15317975

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