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1xvj

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[[Image:1xvj.gif|left|200px]]
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{{STRUCTURE_1xvj| PDB=1xvj | SCENE= }}
{{STRUCTURE_1xvj| PDB=1xvj | SCENE= }}
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'''Crystal Structure Of Rat alpha-Parvalbumin D94S/G98E Mutant'''
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===Crystal Structure Of Rat alpha-Parvalbumin D94S/G98E Mutant===
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==Overview==
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Simultaneous replacement of Asp-94 with serine and Gly-98 with glutamate in rat alpha-parvalbumin creates a CD-site ligand array in the context of the EF-site binding loop. Previous work has shown that, relative to the wild-type CD site, this engineered site has markedly reduced Ca(2+) affinity. Seeking an explanation for this phenomenon, we have obtained the crystal structure of the alpha D94S/G98E variant. The Ca(2+) coordination within the engineered EF site of the 94/98E variant is nearly identical to that within the CD site, suggesting that the attenuated affinity of the EF site in 94/98E is not a consequence of suboptimal coordination geometry. We have also examined the divalent ion binding properties of the alpha 94/98E variant in both Na(+)- and K(+)-containing buffers. Although the Ca(2+) and Mg(2+) affinities are higher in K(+) solution, the increases are comparable to those observed for wild-type alpha. Consistent with that finding, the apparent Na(+) stoichiometry, estimated from stability studies conducted as a function of Na(+) concentration, is 1.0 +/- 0.1, identical to that of wild-type alpha. Thus, the reduced affinity for divalent ions is evidently not the result of heightened monovalent ion competition. The thermodynamic analysis indicates that the less favorable Gibbs free energy of binding reflects a substantial enthalpic penalty. Significantly, the crystal structure reveals a steric clash between Phe-57 and the C(gamma) atom of Glu-98. The consequent displacement of Phe-57 also produces a close contact with Ser-55. Thus, steric interference may be the source of the enthalpic penalty.
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(as it appears on PubMed at http://www.pubmed.gov), where 16101280 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16101280}}
==About this Structure==
==About this Structure==
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[[Category: Ef hand protein]]
[[Category: Ef hand protein]]
[[Category: Parvalbumin]]
[[Category: Parvalbumin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:33:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:36:29 2008''

Revision as of 12:36, 27 July 2008

Image:1xvj.png

Template:STRUCTURE 1xvj

Crystal Structure Of Rat alpha-Parvalbumin D94S/G98E Mutant

Template:ABSTRACT PUBMED 16101280

About this Structure

1XVJ is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the D94S/G98E variant of rat alpha-parvalbumin. An explanation for the reduced divalent ion affinity., Tanner JJ, Agah S, Lee YH, Henzl MT, Biochemistry. 2005 Aug 23;44(33):10966-76. PMID:16101280

Page seeded by OCA on Sun Jul 27 15:36:29 2008

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