This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2f3w

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2f3w.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2f3w.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2f3w| PDB=2f3w | SCENE= }}
{{STRUCTURE_2f3w| PDB=2f3w | SCENE= }}
-
'''solution structure of 1-110 fragment of staphylococcal nuclease in 2M TMAO'''
+
===solution structure of 1-110 fragment of staphylococcal nuclease in 2M TMAO===
-
==Overview==
+
<!--
-
Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease (SNase110) have been studied by various biophysical and NMR methods. Samples of G-88W- and V-66W-mutant SNase110, namely G-88W110 and V-66W110, in aqueous solution and SNase110 in 2.0 M TMAO are adopted in this study. The unfolding transitions and folded conformations of the three SNase fragments were detected by far- and near-ultraviolet circular dichroism and intrinsic tryptophan fluorescence measurements. The tertiary structures and internal motions of the fragments were determined by NMR spectroscopy. Both G-88W and V-66W single mutations as well as a small organic osmolyte (Trimethylamine N-oxide, TMAO) can fold the fragment into a native-like conformation. However, the tertiary structures of the three fragments exhibit different degrees of folding stability and compactness. G-88W110 adopts a relatively rigid structure representing a most stable native-like beta-subdomain conformation of the three fragments. V-66W110- and TMAO-stabilized SNase110 produce less compact structures having a less stable "beta-barrel" structural region. The different folding status accounts for the different backbone dynamic and urea-unfolding transition features of the three fragments. The G-20I/G-29I-mutant variants of the three fragments have provided the evidence that the folding status is correlated closely to the packing of the beta-strands in the beta-barrel of the fragments. The native-like beta-barrel structural region acts as a nonlocal nucleus for folding the fragment. The tertiary folding of the three fragments is initiated by formation of the local nucleation sites at two beta-turn regions, I-18-D-21 and Y-27-Q-30, and developed by the formation of a nonlocal nucleation site at the beta-barrel region. The formation of beta-barrel and overall structure is concerted, but the level of cooperativity is different for the three 1-110 residues SNase fragments.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17172296}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17172296 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17172296}}
==About this Structure==
==About this Structure==
-
2F3W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3W OCA].
+
2F3W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3W OCA].
==Reference==
==Reference==
Line 30: Line 34:
[[Category: Ye, K.]]
[[Category: Ye, K.]]
[[Category: Ob-fold]]
[[Category: Ob-fold]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:26:02 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:43:36 2008''

Revision as of 12:43, 27 July 2008

Image:2f3w.png

Template:STRUCTURE 2f3w

solution structure of 1-110 fragment of staphylococcal nuclease in 2M TMAO

Template:ABSTRACT PUBMED 17172296

About this Structure

2F3W is a Single protein structure of sequence from Staphylococcus aureus. Full experimental information is available from OCA.

Reference

Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease., Xie T, Liu D, Feng Y, Shan L, Wang J, Biophys J. 2007 Mar 15;92(6):2090-107. Epub 2006 Dec 15. PMID:17172296

Page seeded by OCA on Sun Jul 27 15:43:36 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2f3w"
Personal tools