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1uz0

From Proteopedia

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{{STRUCTURE_1uz0| PDB=1uz0 | SCENE= }}
{{STRUCTURE_1uz0| PDB=1uz0 | SCENE= }}
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'''CARBOHYDRATE BINDING MODULE (CBM6CM-2) FROM CELLVIBRIO MIXTUS LICHENASE 5A IN COMPLEX WITH GLC-4GLC-3GLC-4GLC'''
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===CARBOHYDRATE BINDING MODULE (CBM6CM-2) FROM CELLVIBRIO MIXTUS LICHENASE 5A IN COMPLEX WITH GLC-4GLC-3GLC-4GLC===
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==Overview==
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Glycoside hydrolases that release fixed carbon from the plant cell wall are of considerable biological and industrial importance. These hydrolases contain non-catalytic carbohydrate binding modules (CBMs) that, by bringing the appended catalytic domain into intimate association with its insoluble substrate, greatly potentiate catalysis. Family 6 CBMs (CBM6) are highly unusual because they contain two distinct clefts (cleft A and cleft B) that potentially can function as binding sites. Henshaw et al. (Henshaw, J., Bolam, D. N., Pires, V. M. R., Czjzek, M., Henrissat, B., Ferreira, L. M. A., Fontes, C. M. G. A., and Gilbert, H. J. (2003) J. Biol. Chem. 279, 21552-21559) show that CmCBM6 contains two binding sites that display both similarities and differences in their ligand specificity. Here we report the crystal structure of CmCBM6 in complex with a variety of ligands that reveals the structural basis for the ligand specificity displayed by this protein. In cleft A the two faces of the terminal sugars of beta-linked oligosaccharides stack against Trp-92 and Tyr-33, whereas the rest of the binding cleft is blocked by Glu-20 and Thr-23, residues that are not present in CBM6 proteins that bind to the internal regions of polysaccharides in cleft A. Cleft B is solvent-exposed and, therefore, able to bind ligands because the loop, which occludes this region in other CBM6 proteins, is much shorter and flexible (lacks a conserved proline) in CmCBM6. Subsites 2 and 3 of cleft B accommodate cellobiose (Glc-beta-1,4-Glc), subsite 4 will bind only to a beta-1,3-linked glucose, whereas subsite 1 can interact with either a beta-1,3- or beta-1,4-linked glucose. These different specificities of the subsites explain how cleft B can accommodate beta-1,4-beta-1,3- or beta-1,3-beta-1,4-linked gluco-configured ligands.
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(as it appears on PubMed at http://www.pubmed.gov), where 15010454 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15010454}}
==About this Structure==
==About this Structure==
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[[Category: Cbm6]]
[[Category: Cbm6]]
[[Category: Mixed beta1]]
[[Category: Mixed beta1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:53:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:34:14 2008''

Revision as of 13:34, 27 July 2008

Image:1uz0.png

Template:STRUCTURE 1uz0

CARBOHYDRATE BINDING MODULE (CBM6CM-2) FROM CELLVIBRIO MIXTUS LICHENASE 5A IN COMPLEX WITH GLC-4GLC-3GLC-4GLC

Template:ABSTRACT PUBMED 15010454

About this Structure

1UZ0 is a Single protein structure of sequence from Cellvibrio mixtus. Full crystallographic information is available from OCA.

Reference

The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5a in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities., Pires VM, Henshaw JL, Prates JA, Bolam DN, Ferreira LM, Fontes CM, Henrissat B, Planas A, Gilbert HJ, Czjzek M, J Biol Chem. 2004 May 14;279(20):21560-8. Epub 2004 Mar 8. PMID:15010454

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