1vbu

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[[Image:1vbu.gif|left|200px]]
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{{STRUCTURE_1vbu| PDB=1vbu | SCENE= }}
{{STRUCTURE_1vbu| PDB=1vbu | SCENE= }}
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'''Crystal structure of native xylanase 10B from Thermotoga maritima'''
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===Crystal structure of native xylanase 10B from Thermotoga maritima===
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==Overview==
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The crystal structure of xylanase 10B from Thermotoga maritima MSB8 (TmxB), a hyperthermostable xylanase, has been solved in its native form and in complex with xylobiose or xylotriose at 1.8 A resolution. In order to gain insight into the substrate subsite and the molecular features for thermal stability, we compared TmxB with family 10 xylanase structures from nine microorganisms. As expected, TmxB folds into a (beta/alpha)8-barrel structure, which is common among the glycoside hydrolase family 10. The enzyme active site and the environment surrounding the xylooligosaccharide of TmxB are highly similar to those of family 10 xylanases. However, only two xylose moieties were found in its binding pocket from the TmxB-xylotriose complex structure. This finding suggests that TmxB could be a potential biocatalyst for the large-scale production of xylobiose. The result of structural analyses also indicated that TmxB possesses some additional features that account for its thermostability. In particular, clusters of aromatic residues together with a lack of exposed hydrophobic residues are characteristic of the TmxB structure. TmxB has also a significant number of ion pairs on the protein surface that are not found in other thermophilic family 10 xylanases.
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The line below this paragraph, {{ABSTRACT_PUBMED_16247799}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16247799 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16247799}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural basis of the substrate subsite and the highly thermal stability of xylanase 10B from Thermotoga maritima MSB8., Ihsanawati, Kumasaka T, Kaneko T, Morokuma C, Yatsunami R, Sato T, Nakamura S, Tanaka N, Proteins. 2005 Dec 1;61(4):999-1009. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16247799 16247799]
Structural basis of the substrate subsite and the highly thermal stability of xylanase 10B from Thermotoga maritima MSB8., Ihsanawati, Kumasaka T, Kaneko T, Morokuma C, Yatsunami R, Sato T, Nakamura S, Tanaka N, Proteins. 2005 Dec 1;61(4):999-1009. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16247799 16247799]
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Crystallization and preliminary X-ray studies of xylanase 10B from Thermotoga maritima., Ihsanawati, Kumasaka T, Kaneko T, Morokuma C, Nakamura S, Tanaka N, Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1659-61. Epub 2003, Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12925805 12925805]
[[Category: Endo-1,4-beta-xylanase]]
[[Category: Endo-1,4-beta-xylanase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Tanaka, N.]]
[[Category: Tanaka, N.]]
[[Category: Xylanase 10b]]
[[Category: Xylanase 10b]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:20:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:56:08 2008''

Revision as of 13:56, 27 July 2008

Image:1vbu.png

Template:STRUCTURE 1vbu

Crystal structure of native xylanase 10B from Thermotoga maritima

Template:ABSTRACT PUBMED 16247799

About this Structure

1VBU is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Structural basis of the substrate subsite and the highly thermal stability of xylanase 10B from Thermotoga maritima MSB8., Ihsanawati, Kumasaka T, Kaneko T, Morokuma C, Yatsunami R, Sato T, Nakamura S, Tanaka N, Proteins. 2005 Dec 1;61(4):999-1009. PMID:16247799

Crystallization and preliminary X-ray studies of xylanase 10B from Thermotoga maritima., Ihsanawati, Kumasaka T, Kaneko T, Morokuma C, Nakamura S, Tanaka N, Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1659-61. Epub 2003, Aug 19. PMID:12925805

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