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| - | [[Image:1xwo.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1xwo.png|left|200px]] |
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| | {{STRUCTURE_1xwo| PDB=1xwo | SCENE= }} | | {{STRUCTURE_1xwo| PDB=1xwo | SCENE= }} |
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| - | '''crystal structrue of goose delta crystallin'''
| + | ===crystal structrue of goose delta crystallin=== |
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| - | ==Overview==
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| - | Delta-crystallin is a soluble structural protein in avian eye lenses that confers special refractive properties. In the presence of GdmCl (guanidinium chloride), tetrameric delta-crystallin undergoes dissociation via a dimeric state to a monomeric molten globule intermediate state. The latter are denatured at higher GdmCl concentrations in a multi-state manner. In the present study, the X-ray structure of goose delta-crystallin was determined to 2.8 A (1 A=0.1 nm). In this structure the first 25 N-terminal residues interact with a hydrophobic cavity in a neighbouring molecule, stabilizing the quaternary structure of this protein. When these 25 residues were deleted this did not produce any gross structural changes, as judged by CD analysis, but slightly altered tryptophan fluorescence and ANS (8-anilino-1-naphthalenesulphonic acid) spectra. The dimeric form was significantly identified as judged by sedimentation velocity and nondenaturing gradient gel electrophoresis. This mutant had increased sensitivity to temperature denaturation and GdmCl concentrations of 0.3-1.0 M. This protein was destabilized about 3.3 kcal/mol (1 kcal=4.184 kJ) due to N-terminal truncation. After incubation at 37 degrees C N-terminal truncated proteins were prone to aggregation, suggesting the presence of the unstable dimeric conformation. An important role for the N-terminus in dimer assembly of goose delta-crystallin is proposed.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16101585}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16101585 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16101585}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Alpha helix bundle]] | | [[Category: Alpha helix bundle]] |
| | [[Category: Double dimer]] | | [[Category: Double dimer]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:36:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:05:09 2008'' |
Revision as of 14:05, 27 July 2008
Template:STRUCTURE 1xwo
crystal structrue of goose delta crystallin
Template:ABSTRACT PUBMED 16101585
About this Structure
1XWO is a Single protein structure of sequence from Anser anser anser. Full crystallographic information is available from OCA.
Reference
The effect of N-terminal truncation on double-dimer assembly of goose delta-crystallin., Lee HJ, Lai YH, Wu SY, Chen YH, Biochem J. 2005 Dec 15;392(Pt 3):545-54. PMID:16101585
Page seeded by OCA on Sun Jul 27 17:05:09 2008
