2ijy

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{{STRUCTURE_2ijy| PDB=2ijy | SCENE= }}
{{STRUCTURE_2ijy| PDB=2ijy | SCENE= }}
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'''NMR structure ensemble for the reduced DsbA disulphide oxidoreductase from Vibrio Cholerae'''
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===NMR structure ensemble for the reduced DsbA disulphide oxidoreductase from Vibrio Cholerae===
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==Overview==
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We have determined the structure of the reduced form of the DsbA oxidoreductase from Vibrio cholerae. The reduced structure shows a high level of similarity to the crystal structure of the oxidized form and is typical of this class of enzyme containing a thioredoxin domain with an inserted alpha-helical domain. Proteolytic and thermal stability measurements show that the reduced form of DsbA is considerably more stable than the oxidized form. NMR relaxation data have been collected and analyzed using a model-free approach to probe the dynamics of the reduced and oxidized states of DsbA. Akaike's information criteria have been applied both in the selection of the model-free models and the diffusion tensors that describe the global motions of each redox form. Analysis of the dynamics reveals that the oxidized protein shows increased disorder on the pico- to nanosecond and micro- to millisecond timescale. Many significant changes in dynamics are located either close to the active site or at the insertion points between the domains. In addition, analysis of the diffusion data shows there is a clear difference in the degree of interdomain movement between oxidized and reduced DsbA with the oxidized form being the more rigid. Principal components analysis has been employed to indicate possible concerted movements in the DsbA structure, which suggests that the modeled interdomain motions affect the catalytic cleft of the enzyme. Taken together, these data provide compelling evidence of a role for dynamics in the catalytic cycle of DsbA.
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{{ABSTRACT_PUBMED_17585933}}
==About this Structure==
==About this Structure==
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2IJY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IJY OCA].
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2IJY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IJY OCA].
==Reference==
==Reference==
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[[Category: Helical domain insert]]
[[Category: Helical domain insert]]
[[Category: Thioredoxin domain]]
[[Category: Thioredoxin domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:35:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:23:45 2008''

Revision as of 14:23, 27 July 2008

Image:2ijy.png

Template:STRUCTURE 2ijy

NMR structure ensemble for the reduced DsbA disulphide oxidoreductase from Vibrio Cholerae

Template:ABSTRACT PUBMED 17585933

About this Structure

2IJY is a Single protein structure of sequence from Vibrio cholerae. Full experimental information is available from OCA.

Reference

Probing the flexibility of the DsbA oxidoreductase from Vibrio cholerae--a 15N - 1H heteronuclear NMR relaxation analysis of oxidized and reduced forms of DsbA., Horne J, d'Auvergne EJ, Coles M, Velkov T, Chin Y, Charman WN, Prankerd R, Gooley PR, Scanlon MJ, J Mol Biol. 2007 Aug 17;371(3):703-16. Epub 2007 May 31. PMID:17585933

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